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Investigating the Dynamic Properties and Structural Topology of Membrane Protein KCNE3 with EPR Spectroscopy

Mohammed Faleel, Fathima Dhilhani
http://orcid.org/0000-0003-3234-8688
http://rave.ohiolink.edu/etdc/view?acc_num=miami1563886643270804

Abstract Details

2019, Master of Science, Miami University, Chemistry and Biochemistry.
KCNE3 (MiRP2) is a membrane protein that modulates the voltage-gated potassium ion channel alpha subunit KCNQ1. Interaction between these two proteins form a leaky channel that maintains ion homeostasis in the cells. This protein is biologically important since it involves in several fluid and ion homeostasis related diseases. This study mainly focused on probing structural dynamic and topology of KCNE3 in lipid bilayer. Successfully expressed, purified, spin labeled KCNE3 was incorporated into POPC/POPG liposomes. CW-EPR spectral line shape analysis and power saturation techniques were used to study KCNE3 dynamic and topology in lipid bilayer respectively. Inverse central line widths and rotational correlation times of CW-EPR spectra were used to analyze the protein dynamic quantitatively. Topology study of KCNE3 was conducted using power saturation depth parameter data.
Gary Lorigan (Advisor)
Carole Dabney-Smith (Committee Chair)
Rick Page (Committee Member)
Dominik Konkolewicz (Committee Member)
58 p.
Biochemistry; Biophysics; Molecular Biology
Voltage-gated potassium ion Channel; KCNE3; EPR

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Citations

  • Mohammed Faleel, F. D. (2019). Investigating the Dynamic Properties and Structural Topology of Membrane Protein KCNE3 with EPR Spectroscopy [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1563886643270804

    APA Style (7th edition)

  • Mohammed Faleel, Fathima Dhilhani. Investigating the Dynamic Properties and Structural Topology of Membrane Protein KCNE3 with EPR Spectroscopy. 2019. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1563886643270804.

    MLA Style (8th edition)

  • Mohammed Faleel, Fathima Dhilhani. "Investigating the Dynamic Properties and Structural Topology of Membrane Protein KCNE3 with EPR Spectroscopy." Master's thesis, Miami University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=miami1563886643270804

    Chicago Manual of Style (17th edition)

miami1563886643270804
259
© 2019, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.