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AN ANATOMICAL COMPARISON OF WILD TYPE AND HOMEOTIC MUTANT FLOWERS OF CLARKIA TEMBLORIENSIS.pdf (1.9 MB)
ETD Abstract Container
Abstract Header
An Anatomical Comparison of Wild Type and Homeotic Mutant Flowers of Clarkia tembloriensis
Author Info
Obrebski, Chelsea Elizabeth
ORCID® Identifier
http://orcid.org/0000-0002-8715-8884
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=miami1573742923582612
Abstract Details
Year and Degree
2019, Master of Science in Botany, Miami University, Biology.
Abstract
Flowers consist of four whorls of organs (sepals, petals, stamens and carpels), each expressing unique characteristics. In floral homeotic mutants, organs develop abnormally, and floral organs of one whorl express characteristics of another whorl. In the homeotic mutant crinkle petal (cp) of Clarkia tembloriensis, previous investigations demonstrated that cp petal is a hybrid organ combining characteristics of sepals and petals. This investigation extends those studies with a comparative anatomical study of trichomes, stomata, and marginal cells in sepals, WT petals, and cp petals. The aim was to determine if cp petals exhibit a hybrid anatomy of sepals and WT petals. Floral spikes (containing buds 0.6mm, 1.0mm, 1.6mm, and 3.0mm in length), buds at pre-anthesis, and flowers 1-day post anthesis were collected and prepared for SEM or light microscopy. Trichomes and stomata were counted and marginal cells were observed and described in flowers harvested one day post-anthesis. Developmental observations of trichomes, guard cells, and marginal cells were also made in all pre-anthesis bud sizes. The results of this anatomical study demonstrated that cp petals of C. tembloriensis exhibit characteristics of both sepals and WT petals. This provides further support for the hybrid nature of cp petals in Clarkia tembloriensis.
Committee
Nancy Smith-Huerta, PhD (Advisor)
Alfredo Huerta, PhD (Committee Member)
Carolyn Keiffer, PhD (Committee Member)
Pages
76 p.
Subject Headings
Anatomy and Physiology
;
Biology
;
Botany
Keywords
Clarkia tembloriensis
;
Onagraceae
;
homeotic mutant
;
B-type mutation
;
crinkle petal
;
postgenital fusion
;
redifferentiated epidermal cells
;
trichome, stomata
;
petals
;
sepals SEM
;
light microscopy
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Refworks
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Citations
Obrebski, C. E. (2019).
An Anatomical Comparison of Wild Type and Homeotic Mutant Flowers of Clarkia tembloriensis
[Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1573742923582612
APA Style (7th edition)
Obrebski, Chelsea.
An Anatomical Comparison of Wild Type and Homeotic Mutant Flowers of Clarkia tembloriensis.
2019. Miami University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=miami1573742923582612.
MLA Style (8th edition)
Obrebski, Chelsea. "An Anatomical Comparison of Wild Type and Homeotic Mutant Flowers of Clarkia tembloriensis." Master's thesis, Miami University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=miami1573742923582612
Chicago Manual of Style (17th edition)
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Document number:
miami1573742923582612
Download Count:
247
Copyright Info
© 2019, some rights reserved.
An Anatomical Comparison of Wild Type and Homeotic Mutant Flowers of Clarkia tembloriensis by Chelsea Elizabeth Obrebski is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Miami University and OhioLINK.