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20201109_Dissertation_KY.pdf (11.11 MB)

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Assessing and Evaluating Biomarkers and Chemical Markers by Targeted and Untargeted Mass Spectrometry-based Metabolomics

Yang, Kundi
http://orcid.org/0000-0002-6781-4514
http://rave.ohiolink.edu/etdc/view?acc_num=miami1605044640528563

Abstract Details

2020, Doctor of Philosophy, Miami University, Chemistry and Biochemistry.
The features of high sensitivity, selectivity, and reproducibility make liquid chromatography coupled mass spectrometry (LC-MS) the mainstream analytical platform in metabolomics studies. In this dissertation work, we developed two LC-MS platforms with electrospray ionization (ESI) sources, including a LC-triple quadrupole (LC-QQQ) MS platform for targeted metabolomics analysis and a LC-Orbitrap MS platform for untargeted metabolomics analysis, to assess and evaluate either biomarkers or chemical markers in various sample matrices. The main focus of targeted metabolomics platform was to study human gut microbiota and how the gut microbiome responds to therapies. We utilized a LC-QQQ targeted platform for this focus and conducted three projects: (1) to evaluate the effects of four nutrients, mucin, bile salts, inorganic salts, and short chain fatty acids, on the gut microbiome in terms of their impact to the microbial metabolic profiles in vitro, (2) to examine the ability of Lactobacillus acidophilus (LA) to ferment black tea extract (BTE) and to investigate the enhancement of antimicrobial ability of the fermented BTE against Escherichia coli due to the increasing abundant of phenolic compounds in the fermentation process, (3) to determine the modulating effects of the exposure to gastrointestinal (GI) ultrafine particles (UFPs) on gut microbial composition and functions that may lead to a systematic evaluation of the impact of UFPs on host health by using an in vivo murine model. Two additional studies were accomplished by using an LC-Orbitrap untargeted/hybrid platform. In one study we used both LC-QQQ and LC-Orbitrap approaches to analyze polar metabolites from micro anaerobic bacterial culture matrices, to systematically differentiate four Lactobacillus species based on their metabolic profiles. In the other project, the LC-Orbitrap was incorporated with a Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) system to determine whether bourbons could be "fingerprinted" by their chemical compositions.
Michael Crowder (Advisor)
Neil Danielson (Committee Chair)
Xin Wang (Committee Member)
Rick Page (Committee Member)
Scott Hartley (Committee Member)
186 p.
Analytical Chemistry; Biochemistry; Chemistry
targeted metabolomics; untargeted metabolomics; mass spectrometry; gut microbiome; bourbon fingerprint

Recommended Citations

Citations

  • Yang, K. (2020). Assessing and Evaluating Biomarkers and Chemical Markers by Targeted and Untargeted Mass Spectrometry-based Metabolomics [Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1605044640528563

    APA Style (7th edition)

  • Yang, Kundi. Assessing and Evaluating Biomarkers and Chemical Markers by Targeted and Untargeted Mass Spectrometry-based Metabolomics . 2020. Miami University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1605044640528563.

    MLA Style (8th edition)

  • Yang, Kundi. "Assessing and Evaluating Biomarkers and Chemical Markers by Targeted and Untargeted Mass Spectrometry-based Metabolomics ." Doctoral dissertation, Miami University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=miami1605044640528563

    Chicago Manual of Style (17th edition)

miami1605044640528563
411
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This open access ETD is published by Miami University and OhioLINK.