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A Continued Analysis of Iron Acquisition Systems in Actinobacillus Actinomycetemcomitans

Shoemaker, Christopher J
http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177635646

Abstract Details

2007, Bachelor of Arts, Miami University, College of Arts and Sciences - Microbiology.
Actinobacillus actinomycetemcomitans (Aa) is a microaerophilic, gram-negative oral pathogen. Congo red and hemin biding assays showed that all tested strains were able to bind this stain and iron-containing compound, respectively. Inspection of the HK1651 genome indicated that this binding activity could be associated with the presence and expression of hms-like genes, which code for similar functions in other pathogens such as Yersinia pestis. A DF2200N derivative in which hmsF was disrupted by insertion mutagenesis showed a large reduction in hemin and Congo red binding activity. Iron-utilization assays showed no significant difference in the ability of the parental strain and the hmsF insertion derivative to use hemin under chelated conditions. These observations are in agreement with the presence of an hms-like locus in the genome of Aa, which seems to be involved in the interaction of cells with hemin but not the utilization of this compound as an iron source.
Luis Actis (Advisor)
Biology, Microbiology
Actinobacillus actinomycetemcomitans; Heme; Hemin; HmsF

Recommended Citations

Citations

  • Shoemaker, C. J. (2007). A Continued Analysis of Iron Acquisition Systems in Actinobacillus Actinomycetemcomitans [Undergraduate thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177635646

    APA Style (7th edition)

  • Shoemaker, Christopher. A Continued Analysis of Iron Acquisition Systems in Actinobacillus Actinomycetemcomitans. 2007. Miami University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177635646.

    MLA Style (8th edition)

  • Shoemaker, Christopher. "A Continued Analysis of Iron Acquisition Systems in Actinobacillus Actinomycetemcomitans." Undergraduate thesis, Miami University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177635646

    Chicago Manual of Style (17th edition)

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© 2007, all rights reserved.
This open access ETD is published by Miami University Honors Theses and OhioLINK.