The desire to improve the historical disparities of Appalachia provoked the Appalachian Regional Commission (ARC) to construct the Appalachian Developmental Highway System (ADHS). The ADHS has brought about more efficient travel, greater accessibility, and the opportunity for economic growth. However, the purpose of the ADHS, to initiate economic sufficiency, has overshadowed the study of change on the region’s landscape. The combination of remote sensing and GIS technologies supports a methodology for evaluating the spatial expression of growth or physical development along highway corridors. This study applies this methodology to explore land use/land cover change in reference to infrastructure investment along the Ohio portion of the ADHS (Corridor D/State Route 32 in southern Ohio). Landsat images from two time segments, 1976 and 2002, over a 26 year period, were chosen to represent the physical change that has taken place from the height of highway construction to the near-present. The results of this study reveal the pattern of change and the extent of urbanized development along this corridor.