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CHARACTERIZATION OF TWO NOVEL CYTOCHROME P450S INVOLVED IN GRAVITROPISM IN ARABIDOPSIS THALIANA

Withers, John C

Abstract Details

2007, Master of Science (MS), Ohio University, Plant Biology (Arts and Sciences).
Understanding gene expression that occurs during gravitropism is important for studying the processes that link the perception of gravity to the growth response. The gravity persistent signal (gps) 1 mutants of Arabidopsis thaliana showed a “no response” phenotype during gravistimulation experiments. GPS1 encoded CYP705A22, a cytochrome P450 protein of unknown function. Microarray data collected from gravistimulated Arabidopsis root tips indicated that the expression of a family member, CYP705A5, was increased following a gravity stimulus. The no response phenotype of gps1 was successfully rescued by introducing the GPS1 gene into the mutant background. An expression profile was generated for CYP705A22 and CYP705A5 using real-time quantitative PCR, and the data indicated that both genes were up-regulated within minutes of gravity stimulation. Reporter gene constructs that fuse CYP705A5 to the green fluorescent protein (GFP) have shown that CYP705A5 is expressed in the root zone of elongation. Computer modeling of the catalytic domain and screening of potential substrates has generated a list of 130 compounds that may be substrates of CYP705A22 and CYP705A5, and many of the compounds were phenylpropanoid derivatives. Protein expression constructs were created for P450 expression in cell culture to determine the specific biological substrate for each.
Sarah Wyatt (Advisor)
113 p.

Recommended Citations

Citations

  • Withers, J. C. (2007). CHARACTERIZATION OF TWO NOVEL CYTOCHROME P450S INVOLVED IN GRAVITROPISM IN ARABIDOPSIS THALIANA [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187184960

    APA Style (7th edition)

  • Withers, John. CHARACTERIZATION OF TWO NOVEL CYTOCHROME P450S INVOLVED IN GRAVITROPISM IN ARABIDOPSIS THALIANA. 2007. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187184960.

    MLA Style (8th edition)

  • Withers, John. "CHARACTERIZATION OF TWO NOVEL CYTOCHROME P450S INVOLVED IN GRAVITROPISM IN ARABIDOPSIS THALIANA." Master's thesis, Ohio University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187184960

    Chicago Manual of Style (17th edition)