Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


ohiou1187631208.pdf (1.28 MB)

ETD Abstract Container

Abstract Header

Investigation of the Binding of Single-Stranded DNA to Single-Walled Carbon Nanotubes as Studied by Absorbance and Fluorescence Spectroscopy

Heines, Maureen M.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187631208

Abstract Details

2007, Master of Science (MS), Ohio University, Chemistry (Arts and Sciences).
The effect of singled-stranded DNA concentration, single-walled carbon nanotube (SWNT) concentration, DNA base pair length, and pH on SWNT dispersion was investigated to gain a better understanding of how DNA binds to SWNTs. Changes in the near-infrared absorbance and fluorescence spectra were used to determine these effects. At low DNA concentration, very little DNA was bound to the SWNTs leading to poor SWNT dispersion. At higher DNA concentration, more DNA was bound to the SWNTs achieving better dispersion. Increasing the DNA concentration and base pair length resulted in better SWNT dispersion as observed from the increasing structure and intensity in the near-infrared spectra. The opposite effect was observed by increasing the SWNT concentration. An equilibrium binding constant was calculated using the Langmuir model but resulted in an implausible value. Varying the pH of DNA/SWNT samples showed DNA to act similar to SDS as a surfactant except under very basic conditions where the fluorescence intensity diminished above pH 11 for the DNA dispersed samples.
Liwei Chen (Advisor)
98 p.
single-walled carbon nanotubes; DNA; Near-infrared spectroscopy; NIR spectroscopy; Absorbance; Fluorescence

Recommended Citations

Citations

  • Heines, M. M. (2007). Investigation of the Binding of Single-Stranded DNA to Single-Walled Carbon Nanotubes as Studied by Absorbance and Fluorescence Spectroscopy [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187631208

    APA Style (7th edition)

  • Heines, Maureen. Investigation of the Binding of Single-Stranded DNA to Single-Walled Carbon Nanotubes as Studied by Absorbance and Fluorescence Spectroscopy. 2007. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187631208.

    MLA Style (8th edition)

  • Heines, Maureen. "Investigation of the Binding of Single-Stranded DNA to Single-Walled Carbon Nanotubes as Studied by Absorbance and Fluorescence Spectroscopy." Master's thesis, Ohio University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187631208

    Chicago Manual of Style (17th edition)

ohiou1187631208
1,704
© 2007, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.