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Characterization of Three Putative Monoamine Oxidase Genes in Caenorhabditis elegans

Kaushal, Setu
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1218747926

Abstract Details

2008, Master of Science (MS), Ohio University, Biological Sciences (Arts and Sciences).
Monoamine neurotransmitters like dopamine and serotonin regulate neuronal function and behavior in animals. In vertebrates, monoamine oxidase (MAO) degrades monoamines. We investigated the function of three amx genes, putative MAO genes in the nematode Caenorhabditis elegans. We hypothesized that if the amx genes encode MAOs, deletion mutants would have a partial or complete absence of MAO activity, causing an increase in monoamines levels, altered monoamine modulated behaviors and hypersensitivity to exogenous monoamines. Using glyoxylic acid induced fluorescence, we quantified dopamine and serotonin in the mutants but did not find any significant differences. However, the amx mutants exhibited abnormalities in some monoamine modulated behaviors and increased sensitivity to exogenous dopamine and serotonin. Finally and interestingly, we found that Pamx-1::GFP transgenic lines suggested that amx-1 was expressed predominantly in chemosensory neurons.
Janet S Duerr, PhD (Advisor)
Mark Berryman, PhD (Committee Member)
Daweoo Lee, PhD (Committee Member)
96 p.
Biology; Cellular Biology; Molecular Biology
Caenorhabditis elegans; monoamines; monoamine oxidase; behavior; transgenics

Recommended Citations

Citations

  • Kaushal, S. (2008). Characterization of Three Putative Monoamine Oxidase Genes in Caenorhabditis elegans [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1218747926

    APA Style (7th edition)

  • Kaushal, Setu. Characterization of Three Putative Monoamine Oxidase Genes in Caenorhabditis elegans. 2008. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1218747926.

    MLA Style (8th edition)

  • Kaushal, Setu. "Characterization of Three Putative Monoamine Oxidase Genes in Caenorhabditis elegans." Master's thesis, Ohio University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1218747926

    Chicago Manual of Style (17th edition)

ohiou1218747926
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© 2008, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.