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IRX14 and IRX14-LIKE: Two Glycosyl Transferases involved in Glucuronoxylan Biosynthesis in Arabidopsis

Keppler, Brian D.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1268243386

Abstract Details

2010, Master of Science (MS), Ohio University, Plant Biology (Arts and Sciences).
IRX14 and IRX14-LIKE are two closely related glycosyl transferases in the glycosyl transferase 43 (GT43) family of Arabidopsis. IRX14 was initially identified during a coexpression search to identify glycosyl transferases involved in cell wall biosynthesis. A T-DNA insertion mutant for IRX14 results in comparatively minor changes, such as irregular xylem, while a mutation for IRX14L results in no changes. However, an irx14 irx14L double mutant severely affects growth and development with the dwarf plants failing to produce an inflorescence stem. Plants which are homozygous for the mutant version of IRX14 but heterozygous for IRX14L [irx14 irx14L(±)] exhibit an intermediate phenotype, including noticeably smaller leaves, stems, and underdeveloped siliques which contain only a few seeds. Additionally, the T-DNA insertion mutant for IRX14 was found to result in a drought tolerant phenotype. Carbohydrate analysis of total cell wall extracts revealed a reduction in xylose for the irx14 and irx14 irx14L(±) mutants, consistent with a defect in glucuronoxylan biosynthesis. Glucuronoxylan, along with cellulose and lignin, are the major components of secondary cell walls in Arabidopsis. Immunolocalization of xylan with the LM10 antibody revealed a loss of xylan in irx14 mutants and a further reduction in the irx14 irx14L(±) mutants. IRX14L likely functions redundantly with IRX14 in glucuronoxylan biosynthesis, with IRX14 having the more important role in the process.
Allan M. Showalter (Advisor)
96 p.
Botany; Genetics; Molecular Biology
Irregular Xylem; Cell Wall; Glycosyl Transferase; Glucuronoxylan; Drought Tolerance; Arabidopsis

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Citations

  • Keppler, B. D. (2010). IRX14 and IRX14-LIKE: Two Glycosyl Transferases involved in Glucuronoxylan Biosynthesis in Arabidopsis [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1268243386

    APA Style (7th edition)

  • Keppler, Brian. IRX14 and IRX14-LIKE: Two Glycosyl Transferases involved in Glucuronoxylan Biosynthesis in Arabidopsis. 2010. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1268243386.

    MLA Style (8th edition)

  • Keppler, Brian. "IRX14 and IRX14-LIKE: Two Glycosyl Transferases involved in Glucuronoxylan Biosynthesis in Arabidopsis." Master's thesis, Ohio University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1268243386

    Chicago Manual of Style (17th edition)

ohiou1268243386
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© 2010, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.