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Dynamic Biochemical Tissue Analysis of L-selectin Ligands on Colon Cancer Tissues

Carlson, Grady E.

Abstract Details

2012, Master of Science (MS), Ohio University, Biomedical Engineering (Engineering and Technology).
Identification and characterization of molecular markers (MϺ) on cancerous tissue can lead to novel diagnostics and prognostics for cancer. Sialofucosylated MϺ, e.g., sialyl Lewis X and sialyl Lewis A, are over expressed in colon cancers and have been associated with poor cancer prognosis. Mounting evidence suggests sialofucosylated MϺ include E-, P- and L-selectin ligands that mediate cancer metastasis with their selectin counterparts. In this study, immunostaining, the current standard for analysis of MϺ on pathological tissue was employed to detect L-selectin/L-selectin ligand binding. However, immunostains did not engender the biophysical conditions requisite for detection of functional L-selectin/L-selectin ligand interactions. In stark contrast, a novel method of tissue analysis, termed dynamic biochemical tissue analysis (DBTA), probed colon cancer tissues for L-selectin ligands and detected the signature event of selectin/selectin ligand binding, i.e., rolling. Microsphere rolling velocities across defined levels of wall shear stress reflected the force-dependent nature of L-selectin/L-selectin ligand interactions, and revealed that in contrast to immunostains, DBTA provides a distinct detection of functional in situ L-selectin ligands on colon cancer tissues.
Monica Burdick, PhD (Advisor)
Ramiro Malgor, MD (Committee Member)
Shiyong Wu, PhD (Committee Member)
Doug Goetz, PhD (Committee Member)
76 p.

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Citations

  • Carlson, G. E. (2012). Dynamic Biochemical Tissue Analysis of L-selectin Ligands on Colon Cancer Tissues [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1343932605

    APA Style (7th edition)

  • Carlson, Grady. Dynamic Biochemical Tissue Analysis of L-selectin Ligands on Colon Cancer Tissues. 2012. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1343932605.

    MLA Style (8th edition)

  • Carlson, Grady. "Dynamic Biochemical Tissue Analysis of L-selectin Ligands on Colon Cancer Tissues." Master's thesis, Ohio University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1343932605

    Chicago Manual of Style (17th edition)