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Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition

Xiong, Chengkai
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366239912

Abstract Details

2013, Master of Science (MS), Ohio University, Biomedical Engineering (Engineering and Technology).
Epithelial-Mesenchymal Transition (EMT) is a key developmental program that is often activated during cancer invasion and metastasis. In breast cancer, EMT is associated with cancer stem cell properties including expression of the stem cell antigenic profile, over-expression of transcription factors involved in EMT, adhesion to bone marrow endothelium and increased migratory capabilities. Research suggests that transforming growth factor (TGF)-ß and Epidermal Growth Factor (EGF) induces EMT and allows cancer cells to become more stem-like. We have shown that TGF-ß1 alone can partly increase expression of EMT-related transcription factors in BT-20 and MCF-7 cells. Furthermore, EGF alone can induce a stem cell-like phenotype in BT-20 cells and promote adhesion on human umbilical vein endothelial cells (HUVECs) but cannot promote expression of EMT-related transcription factors in BT-20 cells. A combination of EGF and TGF-ß1 can promote expression of EMT-related transcription factors in BT-20 cells. These results indicate that a combination of EGF and TGF-ß1 can induce EMT and cause acquisition of a stem-like phenotype by human breast cancer cell lines.
Fabian Benencia (Advisor)
Douglas Goetz (Committee Member)
Monica Burdick (Committee Member)
Xiaozhuo Chen (Committee Member)
55 p.
Biomedical Engineering
Epithelial-Mesenchymal Transition; stem cell; transforming growth factor; Epidermal Growth Factor; breast cancer

Recommended Citations

Citations

  • Xiong, C. (2013). Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366239912

    APA Style (7th edition)

  • Xiong, Chengkai. Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition. 2013. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366239912.

    MLA Style (8th edition)

  • Xiong, Chengkai. "Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition." Master's thesis, Ohio University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366239912

    Chicago Manual of Style (17th edition)

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© 2013, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.