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An Optimized Polymerase Chain Reaction to Verify the Presence or Absence of the Growth Hormone Receptor Gene

Zhang, Han
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366378898

Abstract Details

2013, Master of Science (MS), Ohio University, Food and Nutrition Sciences (Health Sciences and Professions).
Growth Hormone (GH) is known as a diabetogenic molecule. In excess it can inhibit insulin's action resulting in insulin resistance and diabetes, which can be seen in the GH receptor knockout (GHR-/-) mice since the absence of GH action throughout the whole body improves insulin sensitivity and increases life span. Three tissues that are both insulin and GH sensitive are liver, adipose tissue, and muscle. To determine their individual contributions, three tissue specific GHR disrupted mouse lines have been independently produced: liver-specific, adipose-specific, and muscle-specific GHR-/- mice using the CRE-LOX system. Tissue specific promoters, albumin, aP2, MCK, were utilized to drive the CRE recombinase expression in liver, adipose, and muscle tissues separately. This thesis aimed to validate the gene deletion in specific tissues at the DNA level. The results confirm that liver and muscle specific GHR-/- mice had the deletion of the GHR gene in the liver and muscle tissues, respectively. However, in the adipose specific GHR-/- mice, all tissues, and not just adipose tissue, had evidence of GHR deletion. These findings need to be taken into account when evaluating the phenotype.
Darlene Berryman (Advisor)
Edward List (Committee Member)
Deborah Murray (Committee Member)
125 p.
Aging; Molecular Biology
Growth hormone; growth hormone receptor; growth hormone receptor knock mice; tissue specific growth hormone knockout mice; insulin resistance; longevity.

Recommended Citations

Citations

  • Zhang, H. (2013). An Optimized Polymerase Chain Reaction to Verify the Presence or Absence of the Growth Hormone Receptor Gene [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366378898

    APA Style (7th edition)

  • Zhang, Han. An Optimized Polymerase Chain Reaction to Verify the Presence or Absence of the Growth Hormone Receptor Gene. 2013. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366378898.

    MLA Style (8th edition)

  • Zhang, Han. "An Optimized Polymerase Chain Reaction to Verify the Presence or Absence of the Growth Hormone Receptor Gene." Master's thesis, Ohio University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366378898

    Chicago Manual of Style (17th edition)

ohiou1366378898
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© 2013, some rights reserved.Creative Commons License An Optimized Polymerase Chain Reaction to Verify the Presence or Absence of the Growth Hormone Receptor Gene by Han Zhang is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Ohio University and OhioLINK.