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Fabrication and Utilization of Microfluidic Devices to Study Mechanical Properties of BT-20 and Hs 578T Human Breast Cancer Cells

Burdette, Aaron J.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1408624952

Abstract Details

2014, Master of Science (MS), Ohio University, Biomedical Engineering (Engineering and Technology).
This study aimed to look for differences in observable measures that were related to the mechanical properties of two primary breast cancer cell lines: BT-20 and Hs 578T. Microfluidic channels were designed that mimicked the microvasculature, and cell entry time, morphology and transit velocity were measured. Understanding the differences in mechanical properties may give insight into the mechanisms that allow different types of cancer cells to leave the primary tumor and travel to secondary sites (metastasis). It was found that the two cell lines have different entry times, a measure that has been found to be related to membrane cortical stiffness, with Hs 578T cells having cortical stiffness that is smaller than that for BT-20 cells. When the size of the microchannels was decreased slightly, however, so that the Hs 578T cell nuclei were deformed but not the nuclei of BT-20 cells, the Hs 578T cells were found to have much longer entry times than BT-20 cells. These results demonstrated that there were differences in observed mechanical behaviors between the cell lines that could be diagnostic. It was also found that cell size affects the transit velocities of the cells, with larger cells having decreased velocities. To determine if there were difference in mechanical behaviors for sub-populations within the cell lines with different expression patterns of CD24/44, the cells were sorted based on their expression of those markers. After 12-18 passages, the cells drifted from 4 their original sorted expression profiles, but differences in expression pattern were still measurable with flow cytometry. No significant differences were seen for entry time or elongation index (EI), however a statistically significant difference was seen for transit velocities, demonstrating a possible connection between CD24/44 expression and velocity through the channels.
David Tees (Committee Chair)
Monica Burdick (Committee Member)
122 p.
Biomedical Engineering
Cancer; Microfluidics; microchannel

Recommended Citations

Citations

  • Burdette, A. J. (2014). Fabrication and Utilization of Microfluidic Devices to Study Mechanical Properties of BT-20 and Hs 578T Human Breast Cancer Cells [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1408624952

    APA Style (7th edition)

  • Burdette, Aaron. Fabrication and Utilization of Microfluidic Devices to Study Mechanical Properties of BT-20 and Hs 578T Human Breast Cancer Cells. 2014. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1408624952.

    MLA Style (8th edition)

  • Burdette, Aaron. "Fabrication and Utilization of Microfluidic Devices to Study Mechanical Properties of BT-20 and Hs 578T Human Breast Cancer Cells." Master's thesis, Ohio University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1408624952

    Chicago Manual of Style (17th edition)

ohiou1408624952
359
© 2014, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.