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Distinct Glycosylations Lead to Breast Cancer Cell Adhesion to E-selectin

Liu, Tiantian
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1469633502

Abstract Details

2016, Master of Science (MS), Ohio University, Chemical Engineering (Engineering and Technology).
The majority of deaths caused by breast cancer are due to cancer metastasis. Breast cancer cells express glycoprotein E-selectin ligands that can be recognized and bind to E-selectin expressed by vascular endothelium. The purpose of this study is to define the type(s) of glycosylation(s) leading to E-selectin ligand function of cell surface proteins expressed by breast cancer cells. BT-20 cells were treated with deoxymannojirimycin (DMJ) to prevent N-linked glycosylation of glycoproteins, whereas cells were treated with benzyl-N-acetyl-a-galactosaminide (Bzl-GalNAc) to prevent O-linked glycosylation. Immunoprecipitation, western blot staining with various antibodies, and ¿ow cytometry were used to measure glycosylation influence on E-selectin ligand function[1]. Real-time PCR showed glycosylation inhibitors treatment did not significantly affect BT-20 CD44 expression. Breast cancer cells E-selectin ligand function is affected by N-linked glycosylation of glycoproteins. A mathematical model also was developed to describe BT-20 cell adhesion to E-selectin. These findings will lead to better understanding of cancer metastasis.
Monica Burdick (Advisor)
47 p.
Chemical Engineering
E-selectin; glycosylation; breast cancer; CD44

Recommended Citations

Citations

  • Liu, T. (2016). Distinct Glycosylations Lead to Breast Cancer Cell Adhesion to E-selectin [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1469633502

    APA Style (7th edition)

  • Liu, Tiantian. Distinct Glycosylations Lead to Breast Cancer Cell Adhesion to E-selectin. 2016. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1469633502.

    MLA Style (8th edition)

  • Liu, Tiantian. "Distinct Glycosylations Lead to Breast Cancer Cell Adhesion to E-selectin." Master's thesis, Ohio University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1469633502

    Chicago Manual of Style (17th edition)

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© 2016, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.