Skip to Main Content
 

Global Search Box

 
 
 

ETD Abstract Container

Abstract Header

Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells

Alhumaid, Haidar S.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1470946411

Abstract Details

2016, Master of Science (MS), Ohio University, Chemistry and Biochemistry (Arts and Sciences).
Adhesion of bacteria to human cells is the first step of bacterial infection. It is suspected that the endothelium is where bacterial infection takes place. Endocarditis and meningitis are examples of bacterial infection to endothelial cells. Despite the numerous studies of the infection of endothelial cells, adhesion of bacteria to endothelium is not fully understood. In this study, we investigated the role of cytoprotective NO and cytotoxic ONOO- on the adhesion of E.coli bacteria to human umbilical vein endothelial cells (HUVECs). The collision of bacteria with endothelial cells induces the release of NO and subsequently ONOO-. By using electrochemical nanosensors, we directly measured the concentrations of NO and ONOO- at different concentrations of bacteria (1×107 - 1×109 bacteria/ml). The concentration of NO increased linearly with increasing bacterial concentration. The concentration of ONOO- increased at low concentrations of bacteria from 1×107 to 1×108 bacteria/ml and decreased at high bacterial concentration at 5×108 and 1×109 bacteria/ml. The ratio of [NO]/[ONOO-] at 1×107, 5×107 and 1×108 bacteria/ml indicates that the attachment of bacteria leads to eNOS uncoupling. Fluorescence spectroscopy was also used to determine the adhesion of bacteria at various bacterial concentrations and proved our hypothesis – the adherence of bacteria increases as the number of bacteria increases. Among several eNOS modulators studied here, sepiapterin showed a significant effect on restoring the endothelium and reducing the adhesion of bacteria. The adhesion of bacteria to endothelial cells is most likely to occur when the level of both NO and ONOO- is significantly reduced.
Tadeusz Malinski (Advisor)
72 p.
Chemistry
Nanoanalytical study; Adhesion of bacteria; Endothelial cells; Nitric Oxide; Peroxynitrite

Recommended Citations

Citations

  • Alhumaid, H. S. (2016). Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1470946411

    APA Style (7th edition)

  • Alhumaid, Haidar. Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells. 2016. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1470946411.

    MLA Style (8th edition)

  • Alhumaid, Haidar. "Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells." Master's thesis, Ohio University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1470946411

    Chicago Manual of Style (17th edition)

ohiou1470946411
235
© 2016, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.