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Mycorrhizal Symbiosis in Forest-Grown American Ginseng (Panax quinquefolius), and the Effect of Mycorrhizal Colonization on Root Ginsenoside Concentrations

Filyaw, Tanner R.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1500112365847568

Abstract Details

2017, Master of Science (MS), Ohio University, Environmental Studies (Voinovich).
American ginseng (Panax quinquefolius L.) is a valuable medicinal plant that has been harvested from the forests of eastern North America for over 300 years. Arbuscular Mycorrhizal Fungi (AMF) are symbiotic soil organisms that colonize plant roots and assist in the uptake of water and nutrients. The role of AMF in the production of American ginseng has become a topic of increasing interest, but forest-based research on this subject is limited. This study quantified AMF colonization in forest-grown ginseng roots, resolved the relationship between AMF colonization and root ginsenoside concentrations, and identified species of AMF present in forest production sites. Roots from four production sites were measured for AMF colonization, and ginsenosides Rg1, Re, Rb1, Rc, Rb2, and Rd were quantified by High Performance Liquid Chromatography (HPLC). AMF spores were extracted from soil samples by wet-sieving, and identified morphologically. Results indicate that AMF colonization varied significantly between sites (p < 0.05), but no significant differences in ginsenoside concentrations were resolved between sites (p = 0.104), nor did concentrations vary with the extent of AMF colonization (p = 0.08). Significant inverse relationships between AMF colonization and Rg1 (p < 0.01) were detected, and there was a positive correlation between AMF colonization and Re (p = 0.007). Rhizophagus intraradices was the only species of AMF identified in production sites. Observed differences in total percent colonization between large and small-scale sites suggests that AMF may be influenced by management interventions in WS production. Furthermore, results may also highlight the underlying importance of growing site, with the highest ginsenoside concentrations and root masses observed in sites with differing management characteristics, and differing levels of AMF colonization.
Sarah Davis (Advisor)
83 p.
Agriculture; Alternative Medicine; Biochemistry; Biology; Botany; Ecology; Environmental Studies; Pharmacology; Plant Biology; Plant Sciences
American ginseng; ginseng; Panax quinquefolius; mycorrhizae; arbuscular mycorrhizal fungi; ginsenosides; wild-simulated; forest-grown

Recommended Citations

Citations

  • Filyaw, T. R. (2017). Mycorrhizal Symbiosis in Forest-Grown American Ginseng (Panax quinquefolius), and the Effect of Mycorrhizal Colonization on Root Ginsenoside Concentrations [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1500112365847568

    APA Style (7th edition)

  • Filyaw, Tanner. Mycorrhizal Symbiosis in Forest-Grown American Ginseng (Panax quinquefolius), and the Effect of Mycorrhizal Colonization on Root Ginsenoside Concentrations. 2017. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1500112365847568.

    MLA Style (8th edition)

  • Filyaw, Tanner. "Mycorrhizal Symbiosis in Forest-Grown American Ginseng (Panax quinquefolius), and the Effect of Mycorrhizal Colonization on Root Ginsenoside Concentrations." Master's thesis, Ohio University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1500112365847568

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Ohio University and OhioLINK.