Skip to Main Content
 

Global Search Box

 
 
 

ETD Abstract Container

Abstract Header

Interactions of Engineered Silica Nanoparticles with Cell Membrane Models

Asghari Adib, Ali
http://orcid.org/0000-0002-9178-1660
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1501764587639053

Abstract Details

2017, Master of Science (MS), Ohio University, Biomedical Engineering (Engineering and Technology).
The ubiquity of engineered nanomaterials in industrial and biomedical applications has led to higher chances of exposure, raising concerns regarding their cytotoxicity. In this thesis, interactions of engineered silica nanoparticles (104 ± 5 nm), coated with different surface functional groups [hydroxyl, amine, and polyethylene glycol (PEG) 2K, PEG 5K, PEG 20K], with lipid monolayer and lipid bilayer membrane models are investigated. The membrane models are comprised of an equimolar mixture of sphingomyelin, cholesterol, and 1,2-dioleoyl-sn-glycero-3-phosphocholine. Surface tension, surface topology, dye leakage, and fluorescence anisotropy were monitored to elucidate the role of particle surface properties in their interactions with membrane models. Monolayer studies demonstrated that PEGylated particles penetrate the lipid monolayer, regardless of PEG molecular weight, while amine and hydroxyl-coated particles did not affect monolayer surface properties. In contrast, amine and hydroxyl-coated particles induced a time-dependent leakage in lipid bilayer model, demonstrating a loss of its integrity. Taken together, these results indicate that while nanoparticle surface properties play an important role in their interactions with lipids, clear differences exist between nanoparticle interactions with lipid monolayers and lipid bilayers of the same composition. These results will also help predict the potential toxic effects of engineered nanoparticles on the cell membrane.
Amir Farnoud (Advisor)
88 p.
Biomedical Engineering; Chemical Engineering
Silica nanoparticles; Lipid monolayer; Vesicle

Recommended Citations

Citations

  • Asghari Adib, A. (2017). Interactions of Engineered Silica Nanoparticles with Cell Membrane Models [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1501764587639053

    APA Style (7th edition)

  • Asghari Adib, Ali. Interactions of Engineered Silica Nanoparticles with Cell Membrane Models. 2017. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1501764587639053.

    MLA Style (8th edition)

  • Asghari Adib, Ali. "Interactions of Engineered Silica Nanoparticles with Cell Membrane Models." Master's thesis, Ohio University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1501764587639053

    Chicago Manual of Style (17th edition)

ohiou1501764587639053
664
© 2017, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.