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Multi-marker Metabarcoding Assessment of Biodiversity within Stream Biofilm Communities along an Acid Mine Drainage Recovery Gradient

Wolf, Daniel I., Wolf
http://orcid.org/0000-0001-6517-0498
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou152594729270327

Abstract Details

2018, Master of Science (MS), Ohio University, Plant Biology (Arts and Sciences).
In southeastern Ohio, historical coal mining has exposed streams to acid mine drainage. Active remediation of these streams has proven to be successful for some streams, while others have Not Recovered based on macroinvertebrate assessment. In this study, biofilms were collected from three Not Recovered streams, three Recovered streams, and two Unimpaired streams. The biodiversity was characterized by metabarcoding using four DNA barcode markers and high-throughput amplicon sequencing. Two universal markers (16S and 18S), were sequenced along with an algal specific marker (UPA) and a diatom specific marker (rbcL). Ordination of Bray-Curtis Index calculated from the total operational taxonomic units (OTUs) present in each site showed the Unimpaired and Not Recovered sites were significantly different. Additionally, Shannon index for the rbcL and UPA markers showed significantly lower alpha diversity in Not Recovered streams compared to Unimpaired streams. Further taxonomic investigation revealed a decrease in relative abundance and OTUs of diatoms in Not Recovered streams compared to Recovered and Unimpaired streams including some diatom indicator genera of good water quality. Overall, results from this study describe biofilm diversity during remediation and support previous findings using other methods to assess stream impairment from AMD.
Vis Morgan, PhD (Advisor)
Wyatt Sarah, PhD (Committee Member)
Johnson Kelly, PhD (Committee Member)
87 p.
Biology; Freshwater Ecology; Molecular Biology
Metabarcoding; AMD; Stream Biofilm; Algae; Biomonitoring

Recommended Citations

Citations

  • Wolf, Wolf, D. I. (2018). Multi-marker Metabarcoding Assessment of Biodiversity within Stream Biofilm Communities along an Acid Mine Drainage Recovery Gradient [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou152594729270327

    APA Style (7th edition)

  • Wolf, Wolf, Daniel. Multi-marker Metabarcoding Assessment of Biodiversity within Stream Biofilm Communities along an Acid Mine Drainage Recovery Gradient. 2018. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou152594729270327.

    MLA Style (8th edition)

  • Wolf, Wolf, Daniel. "Multi-marker Metabarcoding Assessment of Biodiversity within Stream Biofilm Communities along an Acid Mine Drainage Recovery Gradient." Master's thesis, Ohio University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou152594729270327

    Chicago Manual of Style (17th edition)

ohiou152594729270327
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© 2018, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.