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The Expression of Cell Surface Heparan Sulfate Proteoglycans and Their Roles in Turkey Skeletal Muscle Formation

Liu, Xiaosong

Abstract Details

2003, Doctor of Philosophy, Ohio State University, Animal Science.
Skeletal muscle myogenesis is a series of highly organized processes that are precisely regulated by the extrinsic environment of muscle cells. Fibroblast growth factor 2 (FGF2) involves in the regulation of myogenesis by stimulating cell proliferation but inhibiting differentiation. As the cell surface heparan sulfate proteoglycans (HSPG), syndecans and glypicans, are the low-affinity receptors of FGF2 and regulate the binding of FGF2 to its high-affinity fibroblast growth factor receptors (FGFR), differences in the expression of these molecules may cause alterations in cell responsiveness to FGF2 stimulation, which may lead to changes in skeletal muscle development and growth. However, the precise functional differences of syndecans and glypicans in FGF2 signaling are unknown to date. Our hypothesis is that syndecans and glypicans may play different roles in regulating the FGF2-FGFR interaction, and the relative expression of these molecules is critical for determining the cell status in proliferation and differentiation. To address how changes in the expression syndecan-1 and glypican-1 are related to FGF2 and FGFR 1 expression and muscle growth properties, a turkey line (F) selected for increased body weight at 16-wk posthatch and its control line (RBC2) were used in this study. The pectoralis major tissue isolated from individuals of the F and RBC2 line at different embryonic and posthatch developmental ages as well as myogenic satellite cells isolated from F and RBC2 line turkeys were used to investigate the expression of syndecan-1, glypican, FGF2, and FGFR 1 during in vivo and in vitro myogenesis. In addition to line differences, the sex differences in syndecan-1, glypican, FGF2, and FGFR 1 expression were also measured. The results showed that in the F line satellite cells the increase in syndecan-1 expression was correlated with the increase in FGF2 expression and cell proliferation as compared to the RBC2 line cells. The expression of syndecan-1 and glypican expression was enhanced in the F line p. major muscle compared to the RBC2 line samples during both embryonic and posthatch development. The results from this study provide novel information about the expression of syndecan-1 and glypican as it relates to FGF2 signaling and skeletal muscle formation.
Sandra Velleman (Advisor)
217 p.

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Citations

  • Liu, X. (2003). The Expression of Cell Surface Heparan Sulfate Proteoglycans and Their Roles in Turkey Skeletal Muscle Formation [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1049125572

    APA Style (7th edition)

  • Liu, Xiaosong. The Expression of Cell Surface Heparan Sulfate Proteoglycans and Their Roles in Turkey Skeletal Muscle Formation. 2003. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1049125572.

    MLA Style (8th edition)

  • Liu, Xiaosong. "The Expression of Cell Surface Heparan Sulfate Proteoglycans and Their Roles in Turkey Skeletal Muscle Formation." Doctoral dissertation, Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1049125572

    Chicago Manual of Style (17th edition)