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osu1070169899.pdf (1.07 MB)
ETD Abstract Container
Abstract Header
Characteristics of
Listeria monocytogenes
Important for Pulsed Electric Field Process Optimization
Author Info
Lado, Beatrice H
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1070169899
Abstract Details
Year and Degree
2003, Doctor of Philosophy, Ohio State University, Food Science and Nutrition.
Abstract
Processing-resistant
Listeria monocytogenes
strains appear sporadically in ready-to-eat food, and constitute a considerable challenge to food processors. Pulsed electric field (PEF) is an alternative to thermal processing and it inactivates pathogens in liquid food. The high-intensity electric pulses kill rapidly microbial contaminants. Since
L. monocytogenes
is among the most PEF-resistant non-sporing foodborne pathogens, this study focused on identifying characteristics of
Listeria
that may be useful for PEF process optimization. Resistance to PEF treatment, at 25 kV/cm, varied among
L. monocytogenes
strains suspended in 0.1% NaCl (pH 7.0) or 50% acid whey (pH 4.2).
Listeria monocytogenes
OSY-8578 was identified as a potential target strain for process optimization, based on its high PEF-resistance. Resistance to PEF could not be associated with genotypic differences, as determined by pulsed field gel electrophoresis or arbitrarily primed-polymerase chain reaction method. However, the target strain, OSY-8578, had a unique genotype, which is potentially useful for its identification. Sublethal PEF process (15 kV/cm for 29 micros) did not induce general stress response, a physiological state with over-expression of major molecular chaperones. On the contrary, expression of the chaperones, GroEL, GroES and DnaJ decreased within 5 to 20 min after PEF treatment. The decrease in expression was more marked for Scott A (PEF-sensitive) than OSY-8578 (PEF-resistant). Interestingly, the chaperone expression profile, after PEF treatment, paralleled an increased sensitivity of the pathogen to mild heat. This sensitization was not observed when mild heating was applied prior to PEF.
Lactobacillus plantarum
ATCC 8014, but not
L. innocua
ATCC 33090, was identified as potential surrogate of the target
L. monocytogenes
, OSY-8578, when these bacteria were suspended in a neutral simple medium (e.g., 0.1% NaCl solution) and treated at 25 kV/cm for 144 micros. Inactivation of
Listeria
by PEF was greater in acid or acidified whey (pH 4.2) than in sweet whey (pH 6.8) or in 0.1% NaCl (pH 7.0). In acid or acidified cheese whey,
Lb. plantarum
was more resistant to PEF than was
L. monocytogenes
OSY-8578; the former bacterium, therefore, may serve as a surrogate of the latter in such media.
Committee
Ahmed Yousef (Advisor)
Pages
257 p.
Keywords
Listeria
;
pulsed electric field
;
target
;
chaperone
;
GroEL
;
GroES
;
DnaJ
;
surrogate
;
sensitization
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Citations
Lado, B. H. (2003).
Characteristics of
Listeria monocytogenes
Important for Pulsed Electric Field Process Optimization
[Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1070169899
APA Style (7th edition)
Lado, Beatrice.
Characteristics of
Listeria monocytogenes
Important for Pulsed Electric Field Process Optimization.
2003. Ohio State University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1070169899.
MLA Style (8th edition)
Lado, Beatrice. "Characteristics of
Listeria monocytogenes
Important for Pulsed Electric Field Process Optimization." Doctoral dissertation, Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1070169899
Chicago Manual of Style (17th edition)
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Document number:
osu1070169899
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Copyright Info
© 2003, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.