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Expression of multiple populations of nicotinic acetylcholine receptors in bovine adrenal chromaffin cells

Wenger, Bryan William
http://rave.ohiolink.edu/etdc/view?acc_num=osu1070496780

Abstract Details

2003, Doctor of Philosophy, Ohio State University, Pharmacy.
The importance of the role of nAChRs in physiological and pathological states is becoming increasingly clear. It is apparent that there are multitudes of nAChR subtypes with different expression patterns, pharmacologies and functions that may be important in various disease states. Therefore, a greater understanding of nAChR subtypes is essential for potential pharmacological intervention in nAChR systems. Bovine adrenal chromaffin cells are a primary culture of a neuronal type cell that express ganglionic types of nAChRs whose activation can be related to a functional response. While much is known about the outcome of functional activation of adrenal nAChRs, little work has been done in characterizing populations of nAChRs in adrenal chromaffin cells. These studies characterize the pharmacology and regulation of populations of nAChRs found in bovine adrenal chromaffin cells. The primary findings of this research include 1) the characterization of an irreversible antagonist of adrenal nAChRs, 2) the discovery of spare nAChRs in bovine adrenal chromaffin cells, 3) the pharmacological characterization of mAb35 insensitive nAChRs which may account for as many as 45% of nAChRs in bovine adrenal chromaffin cells, 4) the finding that disulfide bonds do not play a critical role in the function of adrenal nAChRs which is contrary to their importance in other tissues, 5) the use of (+)-tubocurarine in a receptor protection assay to identify a subpopulation of nAChRs, 6) the characterization of the role of protein synthesis, glycosylation, and phosphorylation in the turnover of nAChRs, and 7) the identification and partial sequencing of a bovine beta4 nAChR transcript expressed in adrenal chromaffin cells.
Dennis McKay (Advisor)
adrenal chromaffin cells; nicotinic acetylcholine receptors; nAChR; spare receptors; ligand gated ion channels; mAb35

Recommended Citations

Citations

  • Wenger, B. W. (2003). Expression of multiple populations of nicotinic acetylcholine receptors in bovine adrenal chromaffin cells [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1070496780

    APA Style (7th edition)

  • Wenger, Bryan. Expression of multiple populations of nicotinic acetylcholine receptors in bovine adrenal chromaffin cells. 2003. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1070496780.

    MLA Style (8th edition)

  • Wenger, Bryan. "Expression of multiple populations of nicotinic acetylcholine receptors in bovine adrenal chromaffin cells." Doctoral dissertation, Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1070496780

    Chicago Manual of Style (17th edition)

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© 2003, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.