Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


osu1110211148.pdf (2.32 MB)

ETD Abstract Container

Abstract Header

Identification and biochemical characterization of a novel receptor:ligand interaction between FcRn and albumin

Chaudhury, Chaity
http://rave.ohiolink.edu/etdc/view?acc_num=osu1110211148

Abstract Details

2005, Doctor of Philosophy, Ohio State University, Ohio State Biochemistry Program.
The inverse relationship between serum albumin concentration and its half-life suggested to early workers that albumin would be protected from a catabolic fate by a receptor mediated mechanism much like that proposed for IgG. This work shows that albumin binds FcRn in a pH dependent fashion, that the lifespan of albumin is shortened in FcRn-deficient mice, and that the plasma albumin concentration of FcRn-deficient mice is less than half that of wild type mice. This study further characterizes the molecular interaction of albumin with FcRn by determining affinity, kinetic rate constants, stoichiometry of interaction and the effect of IgG on the interaction. This study employs western blot analysis, ELISA, animal studies, surface plasmon resonance and isothermal titration calorimetry as experimental tools for these analyses. These results affirm the hypothesis that the MHC-related Fc receptor protects albumin from degradation just as it does IgG, prolonging the half-lives of both.
Clark Anderson (Advisor)
95 p.
Chemistry, Biochemistry
FcRn; HSA; SPR; ITC; ELISA; half-life; dissociation constant

Recommended Citations

Citations

  • Chaudhury, C. (2005). Identification and biochemical characterization of a novel receptor:ligand interaction between FcRn and albumin [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1110211148

    APA Style (7th edition)

  • Chaudhury, Chaity. Identification and biochemical characterization of a novel receptor:ligand interaction between FcRn and albumin. 2005. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1110211148.

    MLA Style (8th edition)

  • Chaudhury, Chaity. "Identification and biochemical characterization of a novel receptor:ligand interaction between FcRn and albumin." Doctoral dissertation, Ohio State University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=osu1110211148

    Chicago Manual of Style (17th edition)

osu1110211148
1,199
© 2005, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.