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Real-time RT-PCR analysis of two epitope regions encoded by the VP2 gene of infectious bursal disease viruses

Mickael, Claudia Silva
http://rave.ohiolink.edu/etdc/view?acc_num=osu1116532082

Abstract Details

2005, Doctor of Philosophy, Ohio State University, Veterinary Preventive Medicine.
Infectious bursal disease virus (IBDV) causes an immunosuppressive disease in chickens and leads to severe economic losses in the poultry industry. Vaccination may not be effective if there is exposure of the vaccinated flock to a different antigenic subtype, which reinforces the importance of identification of new IBDV variants. The virus outer capsid is constituted of VP2, in which the major neutralizing epitopes are located. Forty-eight bursa samples collected from IBDV infected commercial broiler flocks in the US were analyzed by real-time RT-PCR using probes designed for two epitope regions of VP2 denominated minor peak 1 and peak B. It was observed that 23, 48 and 44 samples tested with the minor peak probes Del-E, STC and F15, respectively, had a lower melting temperature (Tm) than expected. Furthermore, 44, 41 and 48 samples tested with the Del-E, STC and F15 peak B probes respectively, had a lower Tm compared to the control, which indicates the presence of one or more nucleotide mutations in the samples. This fact was confirmed by nucleotide sequencing which also demonstrated that most mutations resulted in amino acid substitutions. Real-time RT-PCR can be a useful tool to assist in the development of more effective vaccination strategies. An analysis of immunosuppression induced by infectious bursal disease virus (IBDV) on Campylobacter infection was conducted. IBDV-induced immunosuppression increased C. jejuni colonization and shedding, emphasizing the need for further investigation into the effect of immunosuppression on the pre-harvest incidence of food-borne pathogens.
Daral Jackwood (Advisor)
Infectious bursal disease virus; real-time RT-PCR; identification

Recommended Citations

Citations

  • Mickael, C. S. (2005). Real-time RT-PCR analysis of two epitope regions encoded by the VP2 gene of infectious bursal disease viruses [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1116532082

    APA Style (7th edition)

  • Mickael, Claudia. Real-time RT-PCR analysis of two epitope regions encoded by the VP2 gene of infectious bursal disease viruses. 2005. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1116532082.

    MLA Style (8th edition)

  • Mickael, Claudia. "Real-time RT-PCR analysis of two epitope regions encoded by the VP2 gene of infectious bursal disease viruses." Doctoral dissertation, Ohio State University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=osu1116532082

    Chicago Manual of Style (17th edition)

osu1116532082
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© 2005, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.