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Effect of biotin supplementation on the metabolism of lactating dairy cows

Ferreira, Gonzalo
http://rave.ohiolink.edu/etdc/view?acc_num=osu1141386276

Abstract Details

2006, Doctor of Philosophy, Ohio State University, Animal Science.
Biotin requirements for lactating dairy cows have not been established. Several studies observed that biotin supplementation increases milk yield by dairy cows, although this response was not consistent. The overall objective of this research was to enhance our understanding of when and how biotin supplementation increases milk production by lactating dairy cows. In the first study, we evaluated the urinary excretion of 3-hydroxy-isovaleric acid (3HIA) as a determinant of biotin status. We hypothesized that high-producing cows have a greater need for biotin, and therefore would excrete more 3HIA than low-producing cows. We also hypothesized that biotin supplementation decreases 3HIA excretion. Biotin supplementation increased milk yield in high-producing cows, but not in low-producing cows. Neither production nor biotin supplementation affected the basal urinary excretion of 3HIA. We concluded that urinary excretion of 3HIA is not a sensitive determinant of biotin status in lactating dairy cows. Other measures of biotin status, such as the concentration of avidin-binding substances (ABS) in plasma, milk and urine, were also evaluated. Contrary to our expectations, high-producing and low-producing cows consuming the control diet had similar ABS concentrations in plasma and milk and similar ABS to creatinine ratios in urine. A sensitive indicator of biotin status for lactating dairy cows is still needed. The mechanism by which biotin supplementation increases milk production is not known. In the second study, we hypothesized that biotin supplementation increases the activity and the gene expression of propionyl-CoA carboxylase (PCC) and pyruvate carboxylase (PC) in the liver of high producing dairy cows. Biotin supplementation tended to increase the activity of PC, but did not affect the activity of PCC. The differential response to biotin supplementation suggests that PCC may have a higher priority for biotin than PC. Biotin supplementation did not affect the gene expression of PCC and PC. We concluded that biotin supplementation can affect the activity of biotin-dependent carboxylases in the liver of lactating dairy cows. Whether biotin supplementation increases glucose production in the liver still needs to be evaluated.
William Weiss (Advisor)
135 p.
Biotin; Dairy cows; 3-hydroxyisovaleric acid; Pyruvate carboxylase; Propionyl-CoA carboxylase

Recommended Citations

Citations

  • Ferreira, G. (2006). Effect of biotin supplementation on the metabolism of lactating dairy cows [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1141386276

    APA Style (7th edition)

  • Ferreira, Gonzalo. Effect of biotin supplementation on the metabolism of lactating dairy cows. 2006. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1141386276.

    MLA Style (8th edition)

  • Ferreira, Gonzalo. "Effect of biotin supplementation on the metabolism of lactating dairy cows." Doctoral dissertation, Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=osu1141386276

    Chicago Manual of Style (17th edition)

osu1141386276
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© 2006, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.