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Role of the RB-E2F pathway in embryonic development: implications for paradigms of cell cycle control

Wenzel, Pamela L.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1179341665

Abstract Details

2007, Doctor of Philosophy, Ohio State University, Molecular Genetics.
The Rb-E2F pathway has been the focus of a tremendous body of research, due in large part to its well-recognized role in tumorigenesis. We report in this study a role for Rb in limiting proliferation of trophoblast stem cells during development of the mouse placenta. These findings point to a critical role for Rb at a time when stem cells are beginning to differentiate. We also address the role of Rb binding partners, E2F1, E2F2, and E2F3, in stem and progenitor cell biology in vivo and in vitro. We find that these transcriptional activators are largely dispensable for proliferation in pluripotent stem cells and in many differentiated cell types; however, E2F1-3 do appear to be critical for cell cycle regulation and survival during and after differentiation of specific lineages. Further, we explore the implications of our findings for the current paradigm of E2F function and consider novel roles for E2F that may be largely independent of transcriptional control of cell cycle-regulated gene expression. Appreciating how these genes function in stem cells will be key to understanding how they impact the cell cycle, senescence, self-renewal, proliferation, apoptosis, and differentiation programs during normal development and tumorigenesis.
Gustavo Leone (Advisor)
153 p.
Rb; E2F; Transcription factors; Development; Placenta; Trophoblast; Stem cells; Lens; Apoptosis; Differentiation; Proliferation; Cell cycle

Recommended Citations

Citations

  • Wenzel, P. L. (2007). Role of the RB-E2F pathway in embryonic development: implications for paradigms of cell cycle control [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1179341665

    APA Style (7th edition)

  • Wenzel, Pamela. Role of the RB-E2F pathway in embryonic development: implications for paradigms of cell cycle control. 2007. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1179341665.

    MLA Style (8th edition)

  • Wenzel, Pamela. "Role of the RB-E2F pathway in embryonic development: implications for paradigms of cell cycle control." Doctoral dissertation, Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=osu1179341665

    Chicago Manual of Style (17th edition)

osu1179341665
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© 2007, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.