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The effect of estrogen status on selenium metabolism in female rats

Zhou, Xiaodong

Abstract Details

2007, Doctor of Philosophy, Ohio State University, Human Nutrition and Food Management.
An association between male and female sex hormones and selenium status has been reported in animals and humans. These relationships may be important in the regulation of selenium metabolism and relative to the possible use of selenium as an adjunct for treatment of hormone-related diseases such as breast cancer. The purpose of the first study was to examine the effect of estrogen status on the absorption, tissue distribution and metabolism of orally administered 75Se-selenite. Female Sprague Dawley rats were bilaterally ovariectomized and implanted with either a placebo pellet (OVX) or pellet with estradiol (OVX+E2) at 7 weeks of age. At 12 weeks of age, 60 µCi of 75Se as selenite was orally administered to each rat. Blood and organs were collected 1, 3, 6, and 24h after dosing. Although apparent absorption of 75Se was independent of estrogen status, hormone associated differences of 75Se levels were noted in plasma, RBC, liver, heart, kidney, spleen, brain, and thymus at certain times. Plasma selenoprotein P (SelP) in OVX+E2 group contained a greater percentage of administered 75Se at 3, 6 and 24h after gavage compared to OVX group. 75Se in plasma glutathione peroxidase (GPx) also was greater in OVX+E2 compared to OVX group at 24h. The second aim was to investigate the effect of estrogen status on selenium status in tissues, and on hepatic mRNA levels of SelP and GPx1. Estrogen significantly increased selenium status as measured by selenium concentration and GPx activity in plasma, liver, and brain. Selenium concentration in RBC was also increased by estrogen treatment. Selenium status in kidney and heart was independent of estrogen treatment. Real-time RT-PCR analysis demonstrated that both hepatic SelP and GPx1 mRNA were significantly increased by estrogen treatment. In conclusion, these results suggest that estrogen status affects distribution of ingested selenium in tissue- and time-dependent manners. Expression of hepatic SelP and GPx was regulated by estrogen at both mRNA and protein levels. As SelP has been shown to function as a selenium transporter, estrogen regulation of SelP may play an important role in whole body metabolism of selenium.
Anne Smith (Advisor)
281 p.

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Citations

  • Zhou, X. (2007). The effect of estrogen status on selenium metabolism in female rats [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1179976985

    APA Style (7th edition)

  • Zhou, Xiaodong. The effect of estrogen status on selenium metabolism in female rats. 2007. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1179976985.

    MLA Style (8th edition)

  • Zhou, Xiaodong. "The effect of estrogen status on selenium metabolism in female rats." Doctoral dissertation, Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=osu1179976985

    Chicago Manual of Style (17th edition)