Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


osu1180469450.pdf (1.63 MB)

ETD Abstract Container

Abstract Header

Studies of E. Coli YIDC insertase during membrane protein insertion

Xie, Kun
http://rave.ohiolink.edu/etdc/view?acc_num=osu1180469450

Abstract Details

2007, Doctor of Philosophy, Ohio State University, Molecular, Cellular, and Developmental Biology.
In bacteria, it has been widely recognized that SecYEG translocon is the major translocase used to insert proteins into or export proteins across the membrane bilayer. It has also been found that SecYEG translocase helps proteins adopt their membrane topology and proper conformation. Recent studies have added another pathway into the paradigm. The Oxa1/Alb3/YidC family is a group of evolutionarily conserved proteins existing in mitochondria, chloroplasts, and bacteria. The Oxa1/Alb3/YidC pathway is believed to independently insert proteins into membrane bilayer and facilitate their subunits assembly in the membrane. In addition, the Oxa1/Alb3/YidC proteins can assist the Sec pathway by helping release the membrane proteins from the Sec translocase channel and moving them laterally into the lipid bilayer. In this research, there are two objectives. First, the interaction of YidC with the SecDF components of the SecYEG translocase was studied in details. The specific region responsible for the interaction was evaluated and the importance of YidC-SecDF binding in membrane protein insertion was investigated. Second, the features of the YidC substrates that promote membrane protein insertion were studied. In particular, the hydrophobicity threshold converting a YidC substrate to Sec pathway and the individual contribution of the twenty amino acids for transmembrane insertion was measured.
Ross Dalbey (Advisor)
110 p.
Biology, Cell

Recommended Citations

Citations

  • Xie, K. (2007). Studies of E. Coli YIDC insertase during membrane protein insertion [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1180469450

    APA Style (7th edition)

  • Xie, Kun. Studies of E. Coli YIDC insertase during membrane protein insertion. 2007. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1180469450.

    MLA Style (8th edition)

  • Xie, Kun. "Studies of E. Coli YIDC insertase during membrane protein insertion." Doctoral dissertation, Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=osu1180469450

    Chicago Manual of Style (17th edition)

osu1180469450
597
© 2007, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.