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Dissecting variation in tomato fruit color quality through digital phenotyping and genetic mapping

Darrigues, Audrey
http://rave.ohiolink.edu/etdc/view?acc_num=osu1190125559

Abstract Details

2007, Doctor of Philosophy, Ohio State University, Horticulture and Crop Science.
Color is an important attribute of processing tomatoes. Color and color uniformity are affected by yellow shoulder disorder (YSD), a ripening disorder that results in discoloration of the proximal end tissues of the fruit. We show lycopene content was reduced by 13-24% in juice from non-YSD tomato. Beta-carotene content was reduced by 4-8% in juice from YSD-affected tomato, although this reduction was not statistically significant. Quantification of carotenoid content was more precise by increasing biological replications rather than analytical replications. To assess genetic contributions to YSD, a color measurement module in the Tomato Analyzer software was tested to quantify color and color uniformity from digital images. This approach improved the efficiency of collecting data, provided high correlations with data collected by colorimeter, and improved estimates of genetic contributions to color. With increased precision and accuracy in measuring color, sampling strategies for higher carotenoid content can be optimized and the genetics of color uniformity in tomato can be uncovered. To elucidate the genetics of YSD, molecular markers were developed and exploited for breeding application. An advanced backcross population (BC2) derived from Solanum lycopersicum (OH88119) x S. pimpinellifolium (PI128216) was evaluated for color. Indices were developed to circumvent the highly correlated traits and to simplify the trait complexity based on principal component analysis. These indices capture the essential features of color intensity and color uniformity. The BC2 population was genotyped with 70 markers for QTL analysis. The population was selfed through four generations to generate an inbred backcross population (BC2S4), which was evaluated for the same traits and genotyped with the same markers as the BC2 population. An F2 population and elite varieties were evaluated for color to confirm the QTL. We found QTL for color intensity on chromosomes 2, 8, and 9, and a QTL for color uniformity on chromosome 6. Positive gain under selection was realized for selection by phenotype and by marker-assisted selection (MAS). Higher gains were realized from MAS. Directional selection strategies are being used to further characterize these QTL and evaluate genetic correlations to other fruit quality traits, disease resistance, and yield.
David Francis (Advisor)
192 p.
Agriculture, Agronomy
TOMATO; COLOR; YELLOW SHOULDER DISORDER; LYCOPENE; TOMATO ANALYZER; QTL MAPPING

Recommended Citations

Citations

  • Darrigues, A. (2007). Dissecting variation in tomato fruit color quality through digital phenotyping and genetic mapping [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1190125559

    APA Style (7th edition)

  • Darrigues, Audrey. Dissecting variation in tomato fruit color quality through digital phenotyping and genetic mapping. 2007. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1190125559.

    MLA Style (8th edition)

  • Darrigues, Audrey. "Dissecting variation in tomato fruit color quality through digital phenotyping and genetic mapping." Doctoral dissertation, Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=osu1190125559

    Chicago Manual of Style (17th edition)

osu1190125559
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© 2007, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.