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osu1204581004.pdf (2.79 MB)
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The Host-pathogen Relationship in Rickettsia: Epidemiological Analysis of RMSF in Ohio and a Comparative Molecular Analysis of Four vir genes
Author Info
Carmichael, Jennifer Rose
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1204581004
Abstract Details
Year and Degree
2008, Doctor of Philosophy, Ohio State University, Molecular Genetics.
Abstract
Members of the vector-borne bacterial genus Rickettsia represent an emerging infectious disease threat and have continually been implicated in epidemics worldwide. It is of vital importance to understand the geographical distribution of disease and rickettsial-infected arthropods vectors. In addition, understanding the dynamics of the relationship between rickettsiae and their arthropod hosts will help aid in identifying important factors for virulence. Dermacentor variabilis dog ticks are the main vector in the eastern United States for Rickettsia rickettsii, the etiological agent of Rocky Mountain spotted fever. The frequency of rickettsial-infected ticks and their geographical location in Ohio over the last twenty years was analyzed. The frequency of rickettsial species was found to remain relatively constant (about 20%), but the incidence of R. rickettsii has increased from 6 to 16%. Also, the geographic distribution of rickettsial-positive ticks has expanded, corresponding to a rise of RMSF in these new areas. Type IV secretion system genes, like the vir group, are important for pathogenicity in many pathogens, but have not been analyzed in Rickettsia. Four vir genes, virB8, virB11, virB4, and virD4 were analyzed in Rickettsia amblyommii infected Amblyomma americanum Lone Star ticks from across the Northeast United States. Results showed that important protein motifs are mutated or absent. This evidence suggests the vir genes may not be functional in Rickettsia or they have evolved a novel role. In addition, analysis of virD4 showed that isolates were most similar to Rickettsia conorii and R. rickettsii, two highly pathogenic species. To determine if these genes are important for virulence, a novel qPCR approach was designed to compare the RNA expression in R. amblyommii-infected A. americanum tick and Vero cells. Here, expression of virB8 and virD4 was increased in Vero cells, while virB4 and virB11 had decreased expression. Since rickettsiae cause disease in humans, increases in expression levels in the mammalian Vero cells may be important for pathogenicity. Understanding all levels of disease, from basic biology and epidemiology, analysis of potential virulence genes, and functional analysis via RNA expression, contributes information that leads us closer to determining the process of rickettsial pathogenicity.
Committee
Paul Fuerst (Advisor)
Arthur Burghes (Committee Member)
Gustavo Leone (Committee Member)
Glen Needham (Committee Member)
Pages
206 p.
Subject Headings
Molecular Biology
Keywords
Rickettsia
;
ticks
;
Dermacentor
;
Amblyomma
;
vir gene
;
host-pathogen
;
, qPCR
;
R. amblyommii
Recommended Citations
Refworks
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RIS
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Citations
Carmichael, J. R. (2008).
The Host-pathogen Relationship in Rickettsia: Epidemiological Analysis of RMSF in Ohio and a Comparative Molecular Analysis of Four vir genes
[Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1204581004
APA Style (7th edition)
Carmichael, Jennifer.
The Host-pathogen Relationship in Rickettsia: Epidemiological Analysis of RMSF in Ohio and a Comparative Molecular Analysis of Four vir genes.
2008. Ohio State University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1204581004.
MLA Style (8th edition)
Carmichael, Jennifer. "The Host-pathogen Relationship in Rickettsia: Epidemiological Analysis of RMSF in Ohio and a Comparative Molecular Analysis of Four vir genes." Doctoral dissertation, Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1204581004
Chicago Manual of Style (17th edition)
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Document number:
osu1204581004
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Copyright Info
© 2008, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.