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Investigation Of The Effect Of Low Molecular Weight Peg On Lysozyme Interactions In Solution Using Composition Gradient Static Light Scattering

Gandhi, Shikha
http://rave.ohiolink.edu/etdc/view?acc_num=osu1204599050

Abstract Details

2008, Master of Science, Ohio State University, Chemical Engineering.
Conventional approaches towards protein crystallization are based on predicting favorable solution conditions for crystal formation through trial and error and are expensive. The second virial coefficient (protein-protein), a thermodynamic solution parameter, is a measure of protein interactions in solution, and more importantly- experimentally accessible. It allows a systematic approach for predicting solution conditions favorable to protein crystallization based on solution thermodynamics.Poly-ethylene glycol (PEG) is a commonly used salting-out agent in protein crystallization. Its effect on influencing protein-protein interactions in solution is attributed popularly to a "depletion effect" in the literature. This work provides experimental evidence that low molecular weight PEG induces repulsion between protein molecules, contrary to the predictions of depletion, if a third protein-protein-polymer virial coefficient is considered in the analysis, in addition to protein-protein and protein-polymer second virial coefficients.
Michael Paulaitis (Advisor)
Barbara Wyslouzil (Committee Member)
85 p.
Biophysics; Chemical Engineering
PEG; protein-protein interactions; second virial coefficient

Recommended Citations

Citations

  • Gandhi, S. (2008). Investigation Of The Effect Of Low Molecular Weight Peg On Lysozyme Interactions In Solution Using Composition Gradient Static Light Scattering [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1204599050

    APA Style (7th edition)

  • Gandhi, Shikha. Investigation Of The Effect Of Low Molecular Weight Peg On Lysozyme Interactions In Solution Using Composition Gradient Static Light Scattering. 2008. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1204599050.

    MLA Style (8th edition)

  • Gandhi, Shikha. "Investigation Of The Effect Of Low Molecular Weight Peg On Lysozyme Interactions In Solution Using Composition Gradient Static Light Scattering." Master's thesis, Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1204599050

    Chicago Manual of Style (17th edition)

osu1204599050
1,940
© 2008, some rights reserved.Creative Commons License Investigation Of The Effect Of Low Molecular Weight Peg On Lysozyme Interactions In Solution Using Composition Gradient Static Light Scattering by Shikha Gandhi is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by The Ohio State University and OhioLINK.