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Development of particulate-based EPR oximetry for regional, temporal, and rapid measurements in tissue

Vikram, Deepti S.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1216337271

Abstract Details

2008, Doctor of Philosophy, Ohio State University, Biophysics.
Electron paramagnetic resonance (EPR) oximetry is a useful research technique and a potential clinical tool. The goal of this dissertation was to establish particulate-based EPR oximetry for dynamic pO2 measurements in vivo and widen its applications for cellular and tissue systems. EPR oximetry was compared to a fluorescence technique in an experimental tumor model. The study found EPR oximetry to be a noninvasive, robust and reliable method for repeated measurements in tumors. In addition, the response time of particulate EPR probes was established to be faster than 3.3 ms. Consequently, EPR oximetry was used for measuring temporal oxygen changes in tissue. The study revealed the presence of inherent fluctuations of oxygen in normal and tumor tissue. The frequency of fluctuations was unaltered even if modifiers of oxygen change were used. Lastly, regional pO2 data in mouse and rat hearts were analyzed. A post-processing algorithm was used to determine components in EPR spectra obtained from probe implanted in the infarct heart. Overall, the above developments will help widen the scope of applications for particulate-based EPR oximetry and will further encourage evaluation of the technique as a potential clinical tool for tissue oxygen measurements.
Periannan Kuppusamy (Advisor)
151 p.
Biophysics
EPR oximetry; particulate probes; oxygen probes; oxygen measurement; tissue oxygen; response time of probes

Recommended Citations

Citations

  • Vikram, D. S. (2008). Development of particulate-based EPR oximetry for regional, temporal, and rapid measurements in tissue [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1216337271

    APA Style (7th edition)

  • Vikram, Deepti. Development of particulate-based EPR oximetry for regional, temporal, and rapid measurements in tissue. 2008. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1216337271.

    MLA Style (8th edition)

  • Vikram, Deepti. "Development of particulate-based EPR oximetry for regional, temporal, and rapid measurements in tissue." Doctoral dissertation, Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1216337271

    Chicago Manual of Style (17th edition)

osu1216337271
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© 2008, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.