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An Examination Of The Kintetic, Structural, And Biological Effects Of Zinc On Lactogenic Cytokine Interaction With The Human Prolactin Receptor

Voorhees, Jeffrey L.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1218141658

Abstract Details

2008, Doctor of Philosophy, Ohio State University, Ohio State Biochemistry Program.
Human prolactin, human growth hormone, and human placental lactogen are members of the class 1 cytokine family and are individually capable of binding and biologically activating the human prolactin receptor. Structural and biological work have demonstrated that zinc is directly involved in placental lactogen and growth hormone-receptor complex formation but not in the formation of the prolactin-receptor complex. Concentrations of circulating zinc vary significantly throughout the life of an individual and the role and activity of lactogenic hormones vary similarly. Previous examinations of the role of zinc in hormone-receptor complex formation have largely focused on demonstrating cytokine binding and identifying factors that mitigate it. However, little work has been done to assess the importance of zinc concentration in experimental conditions. This work utilizes surface plasmon resonance (SPR), novel mathematical modeling, FRET, and biological assays to illuminate previous work by directly comparing the effects of zinc on the particular kinetic, conformational, and biological changes in the interaction of lactogentic cytokines with the human prolactin receptor.
Charles L. Brooks, PhD (Advisor)
James A. Cowan, PhD (Committee Member)
Ross Dalbey, PhD (Committee Member)
Donald H. Dean, PhD (Committee Member)
118 p.
Biochemistry
cytokine; prolactin; growth hormone; placental lactogen; SPR

Recommended Citations

Citations

  • Voorhees, J. L. (2008). An Examination Of The Kintetic, Structural, And Biological Effects Of Zinc On Lactogenic Cytokine Interaction With The Human Prolactin Receptor [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1218141658

    APA Style (7th edition)

  • Voorhees, Jeffrey. An Examination Of The Kintetic, Structural, And Biological Effects Of Zinc On Lactogenic Cytokine Interaction With The Human Prolactin Receptor. 2008. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1218141658.

    MLA Style (8th edition)

  • Voorhees, Jeffrey. "An Examination Of The Kintetic, Structural, And Biological Effects Of Zinc On Lactogenic Cytokine Interaction With The Human Prolactin Receptor." Doctoral dissertation, Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1218141658

    Chicago Manual of Style (17th edition)

osu1218141658
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© 2008, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.