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The Effect of Digestive Modification on the Anticancer Activity of Tea Catechins in the HT-29 Human Colon Cancer Cell Line

Cenky, Marti A.

Abstract Details

2009, Master of Science, Ohio State University, Human Nutrition and Food Management.
Epidemiological evidence suggests that increased consumption of catechin-rich tea is associated with reduced risk of gastrointestinal cancers. However, the effects of digestive conditions on the anticancer activity of tea and catechins have been largely ignored. The present study compared the anticancer activities of undigested (raw) and digested tea extracts/catechins in colon cancer cell line HT-29. Cell viability was measured by the MTT assay and the concentration of extracts required to reduce cell viability by 50% (IC50) was determined. Raw material green tea and EGCG extracts showed significantly lower IC50 than the respective digestive compounds. There was no difference in IC50 between raw and digested black tea. Intracellular oxidation was measured by the DCF assay. No difference in intracellular oxidation was observed between raw and digested extracts. These data suggest that digestive conditions impact the anticancer activity of tea catechins; however the mechanism impacted by digestion is not yet known.
Joshua Bomser, PhD (Advisor)
Earl Harrison, PhD (Committee Member)
Steven Schwartz, PhD (Committee Member)
63 p.

Recommended Citations

Citations

  • Cenky, M. A. (2009). The Effect of Digestive Modification on the Anticancer Activity of Tea Catechins in the HT-29 Human Colon Cancer Cell Line [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1235400473

    APA Style (7th edition)

  • Cenky, Marti. The Effect of Digestive Modification on the Anticancer Activity of Tea Catechins in the HT-29 Human Colon Cancer Cell Line. 2009. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1235400473.

    MLA Style (8th edition)

  • Cenky, Marti. "The Effect of Digestive Modification on the Anticancer Activity of Tea Catechins in the HT-29 Human Colon Cancer Cell Line." Master's thesis, Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1235400473

    Chicago Manual of Style (17th edition)