Skip to Main Content
 

Global Search Box

 
 
 
 

Files

ETD Abstract Container

Abstract Header

COMPARATIVE LIPIDOMICS OF HYDROGEL CONTACT LENSES IN-VITRO AND IN-VIVO

Lewis, Kristen Oblad

Abstract Details

2009, Master of Science, Ohio State University, Vision Science.

Contact lenses remain a popular option for the correction of ametropia with an estimated 125 million contact lens wearers throughout the world. Silicone hydrogel lenses, a new contact lens material, allow for more oxygen to reach the ocular surface, the interaction between this hydrophobic material and the hydrophilic layer of the tear film may lead to ocular complications, such as dry eye.

Approximately 80% of problems found in contact lens wearers are caused by molecules from the tear film depositing onto the lenses. Contact lens deposition can cause a decrease in visual acuity, contact lens comfort, and contact lens wettability which leads to an increased inflammatory response and thus dry eye symptoms. In 2003, Jones et al. showed that silicone hydrogel lenses deposited less protein but more lipid compared to traditional hydrogel lenses. To further advance the contact lens field research is needed to compare traditional hydrogel lenses and silicone hydrogel lenses to adequately assess the effect of silicone hydrogel lenses on the ocular surface. The range of analytical methods used to research contact lens deposition needs to be narrowed in order to compare results across studies. The purpose of this study is to analyze lipid deposition on different contact lens materials using assay based techniques and mass spectrometry.

This three arm project consisted of an in-vitro portion followed by two in-vivo studies. The in-vitro study consisted of a 5 mM fatty acid amide doping solution containing oleamide, stearamide, erucamide, palmitamide, and myristamide. Etafilcon A, senofilcon A, and lotrafilcon B were rocked in the doping solution for one week then analyzed by Micromass LCT.

The second arm consisted of five subjects wearing three lenses (etafilcon A, senofilcon A, and lotrafilcon B) for one week of extended wear and analyzed by Micromass LCT.

The third arm of the study and second in-vivo study consisted of 14 subjects wearing etafilcon A, senofilcon A, crofilcon A, and lotrafilcon B each for eight hours then analyzed by mass spectrometry using the Micromass Q-TOF II, a cholesterol assay, a total lipid assay and a phospholipid assay.

Oleamide was the most abundant fatty acid amide present on all contact lenses for the in-vitro study and one week in-vivo study. No specific molecule was identified and no type of contact lens showed an increased amount of deposition from the one day in-vivo study.

The total lipid assay showed that senofilcon A and lotrafilcon B had the highest amount of total lipid. The phospholipid assay indicated that senofilcon A deposited the greatest concentration of phospholipids. Calculations show a large standard deviation for each lens from both assays which indicate individual variation. The cholesterol assay gave no accurate or valuable data.

The results indicate that silicone hydrogel lenses are likely to deposit more lipid molecules in comparison to traditional hydrogel lenses. The total lipid assay and phospholipid assay shows the variation of lipid deposition based on an individual’s tear film chemistry. Further analysis is needed to correctly identify the molecules extracted from worn contact lenses.

Kelly Nichols, PhD (Advisor)
Kari Green-Church, PhD (Committee Member)
Jason Nichols, PhD (Committee Member)
105 p.

Recommended Citations

Citations

  • Lewis, K. O. (2009). COMPARATIVE LIPIDOMICS OF HYDROGEL CONTACT LENSES IN-VITRO AND IN-VIVO [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1243889772

    APA Style (7th edition)

  • Lewis, Kristen. COMPARATIVE LIPIDOMICS OF HYDROGEL CONTACT LENSES IN-VITRO AND IN-VIVO. 2009. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1243889772.

    MLA Style (8th edition)

  • Lewis, Kristen. "COMPARATIVE LIPIDOMICS OF HYDROGEL CONTACT LENSES IN-VITRO AND IN-VIVO." Master's thesis, Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1243889772

    Chicago Manual of Style (17th edition)