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Ferrocyanide: An Inappropriate Reagent for ds-DNA Binding Mode Determination

Burya, Scott J.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1248721621

Abstract Details

2009, Master of Science, Ohio State University, Chemistry.
Determination of the DNA binding mode is an essential step in the development of cytotoxic drugs. Relative quenching of luminescent complexes bound to DNA by ferrocyanide is a method used to show that a complex is intercalated between DNA base pairs. Ferrocyanide quenching experiments were run on three Ru(II) complexes with known ds-DNA binding modes and binding constants in order to validate this method. It was found that the quenching studies were unsuccessful at predicting the binding mode of one of the complexes and this work shows that ferrocyanide quenching efficiency is dependent upon the binding constants of the complexes to DNA, not the binding mode, as was previously believed. Therefore, ferrocyanide quenching studies should not be used to establish an intercalative binding mode, as more suitable methods, such as relative viscosity and DNA melting temperature studies, are available. In addition, this work revealed that dynamic quenching predominates over static quenching for quenching of [Ru(bpy)3]2+ by ferrocyanide in aqueous media.
Claudia Turro, PhD (Advisor)
Christopher Jaroniec, PhD (Committee Member)
53 p.
Analytical Chemistry; Chemistry
ferrocyanide; quenching; hexacyanoferrate; Fe(CN)6; ruthenium; Ru(bpy)3; DNA binding; quenching mechanism; Ru(II); binding mode

Recommended Citations

Citations

  • Burya, S. J. (2009). Ferrocyanide: An Inappropriate Reagent for ds-DNA Binding Mode Determination [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1248721621

    APA Style (7th edition)

  • Burya, Scott. Ferrocyanide: An Inappropriate Reagent for ds-DNA Binding Mode Determination. 2009. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1248721621.

    MLA Style (8th edition)

  • Burya, Scott. "Ferrocyanide: An Inappropriate Reagent for ds-DNA Binding Mode Determination." Master's thesis, Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1248721621

    Chicago Manual of Style (17th edition)

osu1248721621
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© 2009, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.