Skip to Main Content
Frequently Asked Questions
Submit an ETD
Global Search Box
Need Help?
Keyword Search
Participating Institutions
Advanced Search
School Logo
Files
File List
osu1250361867.pdf (1.48 MB)
ETD Abstract Container
Abstract Header
Actions of Secreted Phosphoprotein 1 in the Bovine Corpus Luteum and the Role of Resident T Lymphocytes during Luteolysis
Author Info
Poole, Daniel Heath
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1250361867
Abstract Details
Year and Degree
2009, Doctor of Philosophy, Ohio State University, Animal Sciences.
Abstract
Lymphocytes are present in the fully functional corpus luteum (CL) and have been shown to proliferate during luteal regression due to migration into, as well as proliferation within, the tissue. Secreted phosphoprotein 1 (SPP1) is a multifunctional extracellular matrix protein. It is hypothesized that bovine luteal cells synthesize SPP1, which acts as a paracrine regulator of immune and other cells of the parenchyma during luteal regression. Thus the objectives of the present study were to: 1) Identify SPP1 expression and its regulators throughout the bovine estrous cycle 2) investigate if removal of SPP1 inhibits lymphocyte migration and proliferation 3) determine if luteal function directs specialization of resident T lymphocyte (RTC) subsets, 4) investigate if the expression of proinflammatory and antiinflammatory cytokines of RTC change in response to prostaglandin (PG) F
2alpha
. Corpora lutea (CL) were removed on days 4, 5, 10–12, and 18 of the estrous cycle, and at 0.5, 1, 4, 8 and 12 hours after a luteolytic injection of PGF
2alpha
to evaluate the role of SPP1 in the CL. Additionally, peripheral and resident T lymphocytes from either functional or regressing CL (collected 8 hr after a luteolytic injection of PGF
2alpha
were isolated from whole blood and from dissociated luteal cell suspensions immunomagnetically by positive selection for cell surface proteins (CD2 and gamma delta). Secreted phosphoprotein 1 is temporally regulated throughout the estrous cycle and stimulated in vitro by luteolytic stimulators, PGF
2alpha
and TNF
alpha
. Additionally, these analyses revealed differences in the percentage of regulatory, pro- and antiinflammatory lymphocytes in the resident T lymphocyte population, depending on luteal status. It is concluded that when the CL is fully functional, resident T lymphocytes are predominately antiinflammatory and may serve as regulatory T lymphocytes to facilitate survival and differentiation of various specialized T lymphocyte subsets. However, upon the induction of luteolysis with PGF
2alpha
, the proportion of proinflammatory lymphocytes increases and tolerance toward luteal proteins may be reduced. These data demonstrate a functional shift from antiinflammatory to proinflammatory phenotypes of T lymphocyte subsets isolated from the corpus luteum. Based on these data, it is proposed that lymphocytes encounter a novel means of lymphocyte regulation and/or differentiation within the CL.
Committee
Joy Pate, PhD (Committee Chair)
Michael Day, PhD (Committee Co-Chair)
John Sheridan, PhD (Committee Member)
Sandra Velleman, PhD (Committee Member)
Pages
251 p.
Subject Headings
Agriculture
;
Animals
;
Immunology
Keywords
Corpus Luteum
;
Secreted Phosphoprotein 1
;
Lymphocytes
;
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Poole, D. H. (2009).
Actions of Secreted Phosphoprotein 1 in the Bovine Corpus Luteum and the Role of Resident T Lymphocytes during Luteolysis
[Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1250361867
APA Style (7th edition)
Poole, Daniel.
Actions of Secreted Phosphoprotein 1 in the Bovine Corpus Luteum and the Role of Resident T Lymphocytes during Luteolysis.
2009. Ohio State University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1250361867.
MLA Style (8th edition)
Poole, Daniel. "Actions of Secreted Phosphoprotein 1 in the Bovine Corpus Luteum and the Role of Resident T Lymphocytes during Luteolysis." Doctoral dissertation, Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1250361867
Chicago Manual of Style (17th edition)
Abstract Footer
Document number:
osu1250361867
Download Count:
1,112
Copyright Info
© 2009, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.