Skip to Main Content
 

Global Search Box

 
 
 
 

Files

ETD Abstract Container

Abstract Header

Synthesis and Characterization of Fluorescent Peptide-Dendron Hybrids: Application as in vivo Optical Imaging Probes

Zwilling, Deirdre R.

Abstract Details

2009, Master of Science, Ohio State University, Chemistry.
We have recently synthesized peptide-dendron hybrids based on an intrinsically α-helical alanine-rich sequence that undergoes an α-helix to β-sheet conformational transition1. These peptide-dendron hybrids have also been shown to self-assemble into nanotube aggregates2 and such structures have potential use in drug delivery systems or biomedical applications. Based on the above findings, here we utilize the peptide-dendron hybrids by functionalizing them with a nonpolar ICG analog on the same face as the dendron along with polar functionality, such as RGD peptide sequence on the other side of the peptide. The ability of the peptide-dendron-ICG conjugates to self-assemble and initial in vivo studies for use as optical imaging probes were evaluated. Transmission electron microscopy (TEM) showed the formation of nanotubes and circular dichroism experiments revealed a broad peak at 218 nm which corresponds to β-sheet structure. Preliminary in vivo studies revealed stability and longer circulation in a pancreatic cancer xenograft model for the peptide-dendron-ICG conjugate. In vitro experiments showed higher stability of peptide-dendron-ICG compared with the control peptide. The synthesis, characterization and spectroscopic details of the IGC derivative and the peptide-dendron conjugates are detailed herein. The focus of the future work will be to synthesize tumor targeted fluorescent peptide-dendron conjugates as contrast agents for optical tumor imaging.
Jonathan Parquette, PhD (Advisor)
Christopher Hadad, PhD (Committee Member)
105 p.

Recommended Citations

Citations

  • Zwilling, D. R. (2009). Synthesis and Characterization of Fluorescent Peptide-Dendron Hybrids: Application as in vivo Optical Imaging Probes [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1259690378

    APA Style (7th edition)

  • Zwilling, Deirdre. Synthesis and Characterization of Fluorescent Peptide-Dendron Hybrids: Application as in vivo Optical Imaging Probes. 2009. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1259690378.

    MLA Style (8th edition)

  • Zwilling, Deirdre. "Synthesis and Characterization of Fluorescent Peptide-Dendron Hybrids: Application as in vivo Optical Imaging Probes." Master's thesis, Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1259690378

    Chicago Manual of Style (17th edition)