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Studies of Electrically Conducting Polymers and Biodegradable Polymers for Bone Tissue Engineering

Wu, Jen-Chieh
http://rave.ohiolink.edu/etdc/view?acc_num=osu1261512507

Abstract Details

2009, Doctor of Philosophy, Ohio State University, Chemistry.
In this study, electrically conducting and biodegradable polymer scaffolds were fabricated and tested on bone cells in vitro and in the mice model. The experiments are discussed in Chapter 2 through Chapter 5. Electric field was found to have a positive effect on bone fracture healing.One of the major challenges for this project was to find the right materials to build scaffolds. The materials had to have electrical conductivity, biocompatibility, and processibility. Chapter 2 discusses block copolymers that were designed and synthesized by ring-opening polymerization. These copolymers consisted of polyaniline, polycaprolactone and polylactic acid. They were found to have good solubility in common organic solvents. Cell toxicity tests demonstrated that the copolymers also had good biocompatibility and biodegradability. Fabricating suitable scaffolds for bone cells was another challenge for this project. Chapter 3 discusses three engineering methods that were used to build scaffolds, including electrospinning, printing, and stamping. Polymer fibers were obtained using the electrospinning method. These fibers were soft, flexible, biocompatible, and suitable for soft-tissue engineering. Two-dimensional sulfonated polyaniline-based scaffolds were built with the printing method and then used in experiments, where bone cells were electrically stimulated. Three-dimensional polymer scaffolds were fabricated with the soft-lithography stamping method. These scaffolds with designed patterns had good mechanical properties and uniform shapes. All of these three types of scaffolds derived from the electrospinning, printing, and stamping methods demonstrated good biocompatibility. To evaluate the effect of an electric field on cell proliferation and differentiation, we carried out cell tests using three cells, including the human osteosarcoma (HOS) cells, mice preosteoblast MC3T3-E1 cells, and mice bone marrow stromal cells (BMSC). HOS cells were used to determine suitable electrical stimulating conditions. Chapter 4 discusses the HOS cell growth, the alkaline phosphatase activity, and the mineral deposition that were tested to evaluate the cell proliferation. We found that the best condition was alternate current (AC) with an amplitude of 400 mV and a frequency of 1 kHz. Chapter 5 discusses mice cells, preosteoblast MC3T3-E1 and bone marrow stromal cells that were tested. Both cells showed enhanced cell proliferation and increased alkaline phosphatase activity under electrical stimulation. Furthermore, they showed enhanced mineral deposition, which is a crucial feature of early-stage bone formation. These are important observations, because it is the first time that research has demonstrated that BMSC can differentiate into osteoblast on the biocompatible conducting polymer scaffolds with electrical stimulation. This may have great potential in future biomedical and therapeutic applications, such as facilitating the bone fracture healing process.
Dr. Arthur J. Epstein (Advisor)
Dr. Terry L. Gustafson (Committee Member)
Dr. Christopher P. Jaroniec (Committee Member)
Chemistry

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Citations

  • Wu, J.-C. (2009). Studies of Electrically Conducting Polymers and Biodegradable Polymers for Bone Tissue Engineering [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1261512507

    APA Style (7th edition)

  • Wu, Jen-Chieh. Studies of Electrically Conducting Polymers and Biodegradable Polymers for Bone Tissue Engineering. 2009. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1261512507.

    MLA Style (8th edition)

  • Wu, Jen-Chieh. "Studies of Electrically Conducting Polymers and Biodegradable Polymers for Bone Tissue Engineering." Doctoral dissertation, Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1261512507

    Chicago Manual of Style (17th edition)

osu1261512507
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© 2009, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.