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Brewster Angle Microscopy Study of Model Lung Surfactant Systems at the Air-Water and Air-Physiological Buffer Interfaces

Castada, Hardy Zingalaoa

Abstract Details

2010, Master of Science, Ohio State University, Chemistry.
The ability of the lungs to function normally can be rooted back to its physiological and molecular components. The natural lung surfactants that lines the alveolar walls reduce the surface tension in the alveoli, prevent the collapse of alveolar walls and maintain a large surface area for an easy access of oxygen into the bloodstream. The absence of lung surfactants results in the collapse of the alveoli and eventually causes the lungs to become stiff. This situation impairs the easy exchange of gases to and from the bloodstream and causes life threatening diseases. Different replacement lung surfactants (RLS) are currently used worldwide to supplement the lack of natural lung surfactants, especially in premature babies. RLS vary in their formulations and can be derived from animal or synthetic sources. The results presented in this work demonstrate the behavior of the different lung surfactant model systems on water and physiological buffer subphase acquired through surface pressure-area isotherm and Brewster angle microscopy. Shifting of the surface-pressure area isotherms of all lung surfactant model systems towards higher mean molecular areas when spread on the buffer subphase was observed. Various theories explain such shifting and are explored in this thesis. Furthermore, the fluidizing effect of POPG and condensing effect of PA in their binary and ternary mixtures were observed and reported. Also, the effect of KL4 (a 21 amino acid peptide that is believed to mimic the structure of the surface protein SP-B in natural lung surfactants) in the ternary mixture of DPPC-POPG-PA spread on the buffer subphase was explored and observed to play a part in the formation of more condensed phase in the system.
Heather Allen, PhD (Advisor)
Susan Olesik, PhD (Committee Member)
163 p.

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Citations

  • Castada, H. Z. (2010). Brewster Angle Microscopy Study of Model Lung Surfactant Systems at the Air-Water and Air-Physiological Buffer Interfaces [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1281642097

    APA Style (7th edition)

  • Castada, Hardy. Brewster Angle Microscopy Study of Model Lung Surfactant Systems at the Air-Water and Air-Physiological Buffer Interfaces. 2010. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1281642097.

    MLA Style (8th edition)

  • Castada, Hardy. "Brewster Angle Microscopy Study of Model Lung Surfactant Systems at the Air-Water and Air-Physiological Buffer Interfaces." Master's thesis, Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1281642097

    Chicago Manual of Style (17th edition)