Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


osu1285089805.pdf (4.81 MB)

ETD Abstract Container

Abstract Header

Comparison of Label and Label-free Quantitative Liquid Chromatography Tandem Mass Spectrometry for Protein Biomarker Discovery

Zhao, Bei
http://rave.ohiolink.edu/etdc/view?acc_num=osu1285089805

Abstract Details

2010, Master of Science, Ohio State University, Chemistry.
Mass spectrometry-based protein quantification approaches are powerful tools for biomarker discovery. In this thesis, a spectral counting-based label-free analysis platform and tandem mass tag isobaric labeling quantification platform are described and evaluated for detecting differential protein abundances of an in vivo system in response to UV radiation. Sample preparation and separation of global protein digests were optimized for both label-free and labeling methods. Statistical evaluations such as principal component analysis and Venn diagram were used to evaluate the reproducibility of the analytical system. For spectral counting analysis, a few different normalization and modification methods such as ratioing of normalized spectral counting, normalized spectral abundance factors, and spectral index were applied and the results were compared. Correlations of the results from spectral counting and tandem mass tags labeling were also carried out. Potential protein biomarkers related to DNA damage repair in the in vivo system are proposed based on both labeling and label-free approaches.
Michael Freitas (Advisor)
Susan Olesik (Advisor)
122 p.
Chemistry
mass spectrometry; label-free; tandem mass tag

Recommended Citations

Citations

  • Zhao, B. (2010). Comparison of Label and Label-free Quantitative Liquid Chromatography Tandem Mass Spectrometry for Protein Biomarker Discovery [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1285089805

    APA Style (7th edition)

  • Zhao, Bei. Comparison of Label and Label-free Quantitative Liquid Chromatography Tandem Mass Spectrometry for Protein Biomarker Discovery. 2010. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1285089805.

    MLA Style (8th edition)

  • Zhao, Bei. "Comparison of Label and Label-free Quantitative Liquid Chromatography Tandem Mass Spectrometry for Protein Biomarker Discovery." Master's thesis, Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1285089805

    Chicago Manual of Style (17th edition)

osu1285089805
2,116
© 2010, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.