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Application of a Portable Handheld Infrared Spectrometer for Quantitation of trans Fat in Edible Oils

Birkel, Emily Ann
http://rave.ohiolink.edu/etdc/view?acc_num=osu1305305940

Abstract Details

2011, Master of Science, Ohio State University, Food Science and Technology.
Trans fat has been linked to increased risk of heart disease, and consumption poses a risk to consumers. FDA has mandated inclusion of trans fat on nutritional labeling, which has created a need for accurate, rapid analytical techniques. FTIR spectroscopy has been successfully applied to trans measurement, and advances in multivariate chemometric analysis and handheld FTIR spectrometers are promising for industrial applications. The objective of this study was to develop a methodology for the application of a portable handheld infrared spectrometer for the accurate measurement of trans fat in edible oils. Pure triglycerides were spiked with known levels of trans-trielaidin and spectra of heated samples were collected on a handheld FTIR spectrometer and benchtop FTIR as a reference method. Calibration models were created by measuring height of the second derivative band at 966 cm-1 and by partial least squares regression (PLSR). Predictive accuracy of the models was validated with an independent test set of commercial edible oils also spiked with trielaidin. Calibration models developed using PLSR and linear regression of band heights gave correlation coefficients R2 > 0.98. The handheld spectrometer coupled with the second derivative method did not produce accurate predictions. However, the handheld unit coupled with PLSR multivariate analysis gave standard error of prediction (SEP) of approximately 1%, comparable to values obtained with benchtop systems. This study demonstrates that handheld FTIR spectroscopy coupled with chemometrics is a suitable method for quantitation of trans fat. This combination is promising for in situ analysis of trans fat in edible oils.
Luis Rodriguez-Saona, PhD (Advisor)
John Litchfield, PhD (Committee Member)
Sheryl Barringer, PhD (Committee Member)
81 p.
Food Science
trans fat; FTIR; chemometrics

Recommended Citations

Citations

  • Birkel, E. A. (2011). Application of a Portable Handheld Infrared Spectrometer for Quantitation of trans Fat in Edible Oils [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1305305940

    APA Style (7th edition)

  • Birkel, Emily. Application of a Portable Handheld Infrared Spectrometer for Quantitation of trans Fat in Edible Oils. 2011. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1305305940.

    MLA Style (8th edition)

  • Birkel, Emily. "Application of a Portable Handheld Infrared Spectrometer for Quantitation of trans Fat in Edible Oils." Master's thesis, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1305305940

    Chicago Manual of Style (17th edition)

osu1305305940
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© 2011, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.