Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


osu1306863533.pdf (2.77 MB)

ETD Abstract Container

Abstract Header

A Respiratory Syncytial Virus Replicon That Is Non-Cytotoxic and Capable of Long-Term Foreign Gene Expression

Malykhina, Olga
http://rave.ohiolink.edu/etdc/view?acc_num=osu1306863533

Abstract Details

2011, Doctor of Philosophy, Ohio State University, Integrated Biomedical Science Graduate Program.
Respiratory syncytial virus (RSV) infection of most cultured cell lines causes cell-cell fusion and death. Cell fusion is caused by the fusion (F) glycoprotein and is clearly cytopathic, but other aspects of RSV infection may also contribute to cytopathology. To investigate this possibility, we generated an RSV replicon that lacks all three of its glycoprotein genes and so cannot cause cell-cell fusion or virus spread. This replicon includes a green fluorescent protein gene and an antibiotic resistance gene to enable detection and selection of replicon-containing cells. Adaptive mutations in the RSV replicon were not required for replicon maintenance. Cells containing the replicon could be cloned and passaged many times in the absence of antibiotic selection, with 99% or more of the cells retaining the replicon after each cell division. Transient expression of the F and G (attachment) glycoproteins supported the production of virions that could transfer the replicon into most cell lines tested. Since the RSV replicon is not toxic to these cultured cells and does not affect their rate of cell division, none of the 8 internal viral proteins, the viral RNA transcripts, or the host response to these molecules or their activities are cytopathic. However, the level of replicon genome and gene expression is controlled in some manner, well below that of complete virus and, as such, might avoid cytotoxicity. RSV replicons could be useful for cytoplasmic gene expression in vitro and in vivo, and for screening compounds active against the viral polymerase.
Mark Peeples, PhD (Advisor)
Douglas McCarty, PhD (Other)
Michael Oglesbee, PhD (Other)
Jianrong Li, PhD (Other)
129 p.
Virology
RSV; respiratory syncytial virus; replicon; chronic infection

Recommended Citations

Citations

  • Malykhina, O. (2011). A Respiratory Syncytial Virus Replicon That Is Non-Cytotoxic and Capable of Long-Term Foreign Gene Expression [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1306863533

    APA Style (7th edition)

  • Malykhina, Olga. A Respiratory Syncytial Virus Replicon That Is Non-Cytotoxic and Capable of Long-Term Foreign Gene Expression. 2011. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1306863533.

    MLA Style (8th edition)

  • Malykhina, Olga. "A Respiratory Syncytial Virus Replicon That Is Non-Cytotoxic and Capable of Long-Term Foreign Gene Expression." Doctoral dissertation, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1306863533

    Chicago Manual of Style (17th edition)

osu1306863533
1,401
© 2011, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.