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The Effects of Hyaluronic Acid on Lens Epithelial Cell Migration In Vitro

Haeussler, David John, Jr.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1306875791

Abstract Details

2011, Master of Science, Ohio State University, Veterinary Clinical Sciences.
Purpose. To determine if canine lens epithelial cells (LEC) contain the hyaluronic acid (HA) receptors CD44 and RHAMM, and if HA can impact posterior capsular opacification (PCO) formation in vitro. Methods. Normal (n=10) and cataractous (n=10) canine LEC were evaluated using immunohistochemistry and RT-PCR for expression of CD44 and RHAMM. In vitro models of PCO formation were used to determine if various HA concentrations altered LEC migration. A one-millimeter scratch was created in cultures of confluent canine LEC. Cells were treated with 0, 0.02, 0.012, or 1.0 mg/mL (n=6 per group) of HA dissolved in culture media. Migration of LEC into the scratch was quantified. Mock cataract surgery was performed on canine cadaver eyes. Capsules were re-distended using one of the following (n=6 per group): PBS only, hydroxypropyl methylcellulose viscoelastic, 1.2% HA (12 mg/ml HA) viscoelastic, or 2.0% (20 mg/ml HA) HA viscoelastic. LEC migration within the capsule was evaluated. Results. Normal and cataractous LEC were positive for CD44 and RHAMM protein and mRNA. CD44 showed a statistically significant increase (p<0.01) in mRNA expression in LECs treated with 1.0 mg/ml of HA as compared to the control and 0.02 mg/ml HA Cultured LECs that were treated with 1.0 mg/mL of HA compared to control showed a significantly smaller area devoid of cells (p=0.001) in the scratch test model compared with the control. The number of LECs increased when capsules were treated with 2% HA (20mg/ml HA) viscoelastic as compared to control (p < 0.0109). Conclusions. Canine LEC possess receptors to respond to HA signaling. Treatment with HA significantly increased the rate of LEC migration in a dose dependent manner. CD44 was upregulated in canine LEC after being treated with 1.0mg/ml of HA. RHAMM was slightly upregulated in canine LEC after being treated with 1.0mg/ml of HA. Viscoelastics containing HA promoted LEC migration and proliferation in vitro. The introduction of exogenous HA in the form of viscoelastics during cataract surgery may promote LEC migration and contribute to PCO formation in vivo.
Heather Chandler, PhD (Advisor)
Anne Gemensky Metzler, DVM (Advisor)
David Wilkie, DVM (Committee Member)
76 p.
Veterinary Services
hyaluronic acid; viscoelastic; lens epithelial cell; cataract

Recommended Citations

Citations

  • Haeussler, Jr., D. J. (2011). The Effects of Hyaluronic Acid on Lens Epithelial Cell Migration In Vitro [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1306875791

    APA Style (7th edition)

  • Haeussler, Jr., David. The Effects of Hyaluronic Acid on Lens Epithelial Cell Migration In Vitro. 2011. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1306875791.

    MLA Style (8th edition)

  • Haeussler, Jr., David. "The Effects of Hyaluronic Acid on Lens Epithelial Cell Migration In Vitro." Master's thesis, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1306875791

    Chicago Manual of Style (17th edition)

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© 2011, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.