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Application of Handheld Infrared Spectrometer for Screening Quality Traits in Tomato (Lycopersicon esculentum Mill.) Cultivars

Sayajon, Glynda Fe Garela
http://rave.ohiolink.edu/etdc/view?acc_num=osu1306941612

Abstract Details

2011, Master of Science, Ohio State University, Food Science and Technology.
Tomatoes are classified for use as fresh or processed tomato products based on their sugar and acid profile. Current methods to analyze sugars and acids content are time and labor intensive making efficient assays for detection and quantification desirable. The ability to rapidly estimate important components related to fruit quality can facilitate efficient selection of tomato breeding-lines and enhanced processing conditions for the development of products with improved quality and health benefits for consumers. Our objective was to develop simple protocols to determine sugars, organic acids and ascorbic acid using a handheld portable Attenuated Total Reflectance Infrared (ATR-IR) spectrometer combined with multivariate analysis. Samples (80) were obtained from genetically diverse tomato varieties that encompassed commercial hybrids, experimental hybrids, and elite parents used in tomato processing and fresh market industry. Tomatoes were blended; aliquots (5 mL) were centrifuged and infrared spectrum was collected from supernatant (10 μL) by applying it directly to the ATR-ZnSe crystal. Reference methods included soluble solids (refractometer), pH, titratable acidity, and HPLC analysis for sugars and acids. Partial least squares regression (PLSR) was used to create calibration models that correlated the tomato components with infrared spectra. Multivariate models accurately predicted all quality attributes with a mean correlation value, R2 >0.84. Performance statistics showed prediction errors (SECV) of ~0.4 for estimation of °Brix and sugars (fructose, glucose (% w/w)), 0.06 for pH and TA (g citric acid/100ml), 0.15 g/100g for citric acid and 1.7 mg/100g ascorbic acid using the infrared region of 1800 - 900 cm-1. The portable handheld ATR-IR spectrometer could provide the tomato industry with an on-field, simple and high throughput method for chemical profiling of tomatoes that could lead to improved varieties with enhanced characteristics for industry and consumer demands.
Luis Enrique Rodriguez-Saona, PhD (Advisor)
Sheryl Barringer, PhD (Committee Member)
Michael Mangino, PhD (Committee Member)
104 p.
Food Science
Tomatoes; chemometrics; infrared; quality assurance

Recommended Citations

Citations

  • Sayajon, G. F. G. (2011). Application of Handheld Infrared Spectrometer for Screening Quality Traits in Tomato (Lycopersicon esculentum Mill.) Cultivars [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1306941612

    APA Style (7th edition)

  • Sayajon, Glynda Fe. Application of Handheld Infrared Spectrometer for Screening Quality Traits in Tomato (Lycopersicon esculentum Mill.) Cultivars. 2011. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1306941612.

    MLA Style (8th edition)

  • Sayajon, Glynda Fe. "Application of Handheld Infrared Spectrometer for Screening Quality Traits in Tomato (Lycopersicon esculentum Mill.) Cultivars." Master's thesis, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1306941612

    Chicago Manual of Style (17th edition)

osu1306941612
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This open access ETD is published by The Ohio State University and OhioLINK.