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Modulation of Protein Tyrosine Phosphatase (PTPγ) Signaling by MicroRNA and Ligand Binding

Lin, Shu-Hong
http://rave.ohiolink.edu/etdc/view?acc_num=osu1312311825

Abstract Details

2011, Master of Science, Ohio State University, Veterinary Biosciences.
Protein Tyrosine Phosphatase γ (PTPγ, encoded by PTPRG gene) has been shown to be an estrogen-regulated tumor suppressor in breast cancers. Our current studies focus on mechanisms which are influential in PTPγ in both breast cancer cell line and primary cultured breast cancer cells. In search for the differences in micorRNA (miRNA) profiles among primary cultured human breast cancer cell (CEC) and adjacent normal cell (NAE), we found 24 up-regulated and 1 down-regulated miRNA in CEC. Among the up-regulated miRNAs, miR-141 is predicted to target PTPRG based on sequence and transient transfection of the precursor for miR-141 (pre-miR-141) in triple negative human breast cancer cell line, MDA-MB-231 cells successfully resulted in the down-regulation of PTPRG. Further experiments with 14 pairs of CEC and NAE from patients revealed a moderate negative association (Kendall’s τ = -0.61) in NAE but not CEC. PTPγ belongs to the receptor type phosphatase family, yet its ligand has not been reported until 2010. Members of contactin (CNTN) family, including CNTN3, 4, 5 and 6, were found to bind PTPγ. However, it is still unclear whether CNTN binding is stimulatory or inhibitory to PTPγ activity. Among the four possible ligands, only CNTN3 was found to be present in normal breast cells. In order to evaluate the impact of CNTN3 binding on PTPγ signaling, we used genes regulated by PTPγ as an indicator. Compared with PTPRG expression level, CNTN3/PTPRG ratio has been found to be equally and even better associated with the expression of downstream genes in CEC and NAE in an opposite direction. Such difference in the direction of association has suggested an inhibitory role for CNTN3 binding in terms of PTPγ signaling.
Young C. Lin (Advisor)
Robert Lee (Committee Member)
McDonald Wick (Committee Member)
59 p.
Biomedical Research
PTPRG; CNTN3; CONTACTIN; Protein Tyrosine Phosphatase; MicroRNA; BREAST CANCER

Recommended Citations

Citations

  • Lin, S.-H. (2011). Modulation of Protein Tyrosine Phosphatase (PTPγ) Signaling by MicroRNA and Ligand Binding [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1312311825

    APA Style (7th edition)

  • Lin, Shu-Hong. Modulation of Protein Tyrosine Phosphatase (PTPγ) Signaling by MicroRNA and Ligand Binding. 2011. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1312311825.

    MLA Style (8th edition)

  • Lin, Shu-Hong. "Modulation of Protein Tyrosine Phosphatase (PTPγ) Signaling by MicroRNA and Ligand Binding." Master's thesis, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1312311825

    Chicago Manual of Style (17th edition)

osu1312311825
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© 2011, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.