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Synthesis and Studies into Conformation, Self-Assembly and Applications of Peptide-Dye Conjugates

Forties, Christina E.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1313528098

Abstract Details

2011, Doctor of Philosophy, Ohio State University, Chemistry.
The ability to design self-assembling peptides that form specic nanostructures has grown signicantly in the past 30 years, so much that the construction of the assemblies have been applied toward applications that span nanotechnology and medicine. Peptide systems alone have the potential to vastly change medicine and technology as we continue to learn more about the structures that are formed and how they can be modied for further applications. Additionally, molecules that are fluorescent in the near-infrared range are important for the rening of photodyamic therapy and near-infrared (NIR) detection for diagnosis of disease. When peptide assemblies combine with fluorescent molecules, a dynamic nanostructure can be envisioned. With the possibility of the peptide assembly to carry a guest molecule, the three dimensional assembly can be tracked and directed through the body to monitor the carry and release process. In light of these ideas and the potential they hold for improvement of drug delivery and detection, a peptide design evolved as inspired by the work of Shao and Parquette (The Ohio State University), where short dilysine peptide systems were functionalized with a π-sheet stacking molecule and self-assembled to nanobrils and ribbons. The work outlined here describes several similar systems that are modified with an indocyanine green derivative that was thought to be a π-sheet stacking initiator to drive self-assembly, while also acting as a NIR agent, in hopes of fashioning structures of both efficiency and practicality. Despite the attempts, the peptide systems investigated did not show evidence of organized secondary structure or nanostructures that was observed in earlier self-assemblies, including Shao’s work. It was hypothesized that the dye might be negatively affecting the self-assembly of the short peptides; one possibility of the dye’s interference includes the introduction of an additional positive charge on the indolenine nitrogen that provides an imbalance of charge, and thus an impetus to self-assembly. Another possibility includes the known strength of the ICG dye to aggregate with itself, which may be overriding the self-assembly of the short peptides systems to β-sheets, and higher order structures. These potenital effects may prevent organization and aggregation; however, aggregation to non-specic type structures was observed via TEM analysis for many of the peptide-dye conjugates, which may be due to amphiphilic-like self-assembly, in addition to dye self-aggregation.
Jonathan Parquette, PhD (Advisor)
Jovica Badjic, PhD (Committee Member)
Christopher Hadad, PhD (Committee Member)
Frederick Villamena, PhD (Committee Member)
261 p.
Chemistry
Peptides; self-assembly; indocyanine dyes

Recommended Citations

Citations

  • Forties, C. E. (2011). Synthesis and Studies into Conformation, Self-Assembly and Applications of Peptide-Dye Conjugates [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1313528098

    APA Style (7th edition)

  • Forties, Christina. Synthesis and Studies into Conformation, Self-Assembly and Applications of Peptide-Dye Conjugates. 2011. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1313528098.

    MLA Style (8th edition)

  • Forties, Christina. "Synthesis and Studies into Conformation, Self-Assembly and Applications of Peptide-Dye Conjugates." Doctoral dissertation, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1313528098

    Chicago Manual of Style (17th edition)

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© 2011, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.