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Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins

Baldauff, Rachel Michelle
http://rave.ohiolink.edu/etdc/view?acc_num=osu1321986125

Abstract Details

2011, Master of Science, Ohio State University, Chemistry.
Overwhelming evidence suggests that low levels of high-density lipoproteins (HDL) increase one’s odds of developing coronary artery disease (CAD). This phenomenon could be due in part to the hydrolase activity of the enzyme human serum paraoxonase-1 (huPON1) that is found bound to the HDL in the circulatory system. Many studies have indicated lowered atherogenic effects due to raised huPON1/HDL levels. In order to fully understand these correlations, more insight into how the interaction of huPON1 and HDL may be delivering its beneficial properties is needed. In the current study, the interactions between reconstituted HDL (rHDL) particles and fluorophore-labeled variants of PON1 (G3C9-ybbR and five other variants) were probed via fluorescence anisotropy, effects on arylesterase and paraoxonase activities, and changes in arylesterase activity upon binding to rHDL. While the mutations to the hydrophobic signal sequence (L12A and L12K) did not appear to have significant effects on the arylesterase or paraoxonase activities, the mutations on the active site lid (W194A and W194K) caused increases in both activities. While the fluorescence anisotropy measurements were unable to reveal anything about the binding affinity, the arylesterase stimulation due to rHDL binding did.
Thomas Magliery, PhD (Advisor)
Terry Gustafson, PhD (Advisor)
98 p.
Biochemistry
rePON1; rHDL; G3C9

Recommended Citations

Citations

  • Baldauff, R. M. (2011). Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1321986125

    APA Style (7th edition)

  • Baldauff, Rachel. Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins. 2011. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1321986125.

    MLA Style (8th edition)

  • Baldauff, Rachel. "Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins." Master's thesis, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1321986125

    Chicago Manual of Style (17th edition)

osu1321986125
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© 2011, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.