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Decontamination of Food Processing Equipment Contaminated with Biofilm-forming Pseudomonas spp. by Ozone-based Cleaning-in-place

Tirpanci, Goksel
http://rave.ohiolink.edu/etdc/view?acc_num=osu1322666028

Abstract Details

2011, Master of Science, Ohio State University, Food Science and Nutrition.
Formation of microbial biofilm on equipment surfaces may cause the recontamination of processed food products. Removal of biofilm from these surfaces by conventional cleaning operations is challenging. The goal of this study was to determine the feasibility of using an ozone-based cleaning-in-place (CIP) system to improve sanitization in food processing facilities. A pilot-scale CIP system, using ozone in the final sanitization step, was successfully constructed. The CIP system was coupled to food processing equipment consisting of a vessel, a filler, a by-pass loop and a 25-feet long pipeline. The processing equipment was contaminated with Pseudomonas spp. in diluted (10 %) sterilized skim milk. Biofilm were developed in equipment by circulating the culture for 3 or 5days, followed by quiescent incubation for 2 days. This equipment was treated with a standard CIP regime consisting of pre-rinse with tap water at ambient temperature (25°C), and alkaline cleaning (0.2% - 0.5% of potassium hydroxide) at 50°C. This standard CIP process was followed by an ozone sanitization step (5 ppm or 10 ppm, at 25°C). Samples of circulating fluids (containing planktonic cells) and surface swabs (representing biofilm bacteria) were taken at different stages of the CIP operation. There were significant reductions in the planktonic populations after prerinsing, however biofilm populations decreased significantly only after alkaline cleaning. Results from swab samples, after the CIP operation, proved that 5 ppm ozone treatment for 3 min or 5 min may not decontaminate food contact surfaces, but 10 ppm ozone treatment for 10 min was able to remove biofilm from these surfaces. With specified cleaning conditions, various surfaces, including the by-pass line and pipeline were sterile; though a part of the filler and gasket were not sterile. The CIP process needs to be fine-tuned to decontaminate some of the hard-to-clean locations, particularly when a more robust biofilm is developed at these locations. This study generally showed that ozone may be used to decontaminate hard-to-clean food processing equipment having biofilm.
Ahmed Yousef (Advisor)
James Harper (Committee Member)
V.M. Balasubramaniam (Committee Member)
Food Science
Ozone; CIP; biofilm; sanitization

Recommended Citations

Citations

  • Tirpanci, G. (2011). Decontamination of Food Processing Equipment Contaminated with Biofilm-forming Pseudomonas spp. by Ozone-based Cleaning-in-place [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1322666028

    APA Style (7th edition)

  • Tirpanci, Goksel. Decontamination of Food Processing Equipment Contaminated with Biofilm-forming Pseudomonas spp. by Ozone-based Cleaning-in-place. 2011. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1322666028.

    MLA Style (8th edition)

  • Tirpanci, Goksel. "Decontamination of Food Processing Equipment Contaminated with Biofilm-forming Pseudomonas spp. by Ozone-based Cleaning-in-place." Master's thesis, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1322666028

    Chicago Manual of Style (17th edition)

osu1322666028
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This open access ETD is published by The Ohio State University and OhioLINK.