Skip to Main Content
Frequently Asked Questions
Submit an ETD
Global Search Box
Need Help?
Keyword Search
Participating Institutions
Advanced Search
School Logo
Files
File List
osu1338376024.pdf (1.54 MB)
ETD Abstract Container
Abstract Header
Immune Mediators in Gingival Crevicular Fluid as Predictors of Healing Outcomes in Re-Implanted Permanent Incisors – A Pilot Investigation
Author Info
Ashley, Amanda
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1338376024
Abstract Details
Year and Degree
2012, Master of Science, Ohio State University, Dentistry.
Abstract
Permanent tooth avulsion is a serious problem in pediatric dentistry. Avulsed permanent incisors that ankylose after re-implantation have profound detrimental long-term consequences in growing patients. Ankylosed and normally healed teeth exhibit significant differences in the levels of growth factors, chemokines and cytokines following traumatic avulsion. This study examines immune mediators including Interleukin 1-Beta, Interleukin-8, Interleukin-10, Interleukin-12, GM-CSF, and VEGF present in the gingival crevicular fluid of avulsed and re-implanted teeth. Gingival crevicular fluid (GCF) collection is a reliable way of monitoring biomarkers around a tooth. GCF composition reflects the physiological status of the periodontal ligament, giving us a window into the ongoing healing process within the socket. GCF collection is rapid, painless, non-invasive, economical, and yields findings that are reproducible. Dental research has historically focused on extra-oral time and storage medium for avulsed teeth as the primary determinants of healing. Pediatric dentists focus on quick re-implantation of the avulsed tooth, proper splinting with a non-rigid splint, antibiotic coverage and pulp extripation within 7-10 days. However, the patient’s own phenotypic variation of biomarker expression may be the determining factor in predicting healing outcomes of avulsed teeth. In this study, interleukin-1-beta (IL-1β) and vascular endothelial growth factor (VEGF) were significantly elevated in GCF collected from patients with re-implanted teeth that healed compared to teeth that ankylosed. These data suggest that avulsed re-implanted teeth elicit pro-inflammatory signals and growth factors in the GCF that could be used as predictive biomarkers. Increased osteoclastic activity within the sockets of re-implanted teeth in combination with increase inflammation likely prevents ankylosis. Instead of trying to maintain periodontal ligament vitality via storage medium, newer treatment approaches may try to alter the patient’s own immune response to the avulsion, preventing detrimental ankylosis and promoting normal healing.
Committee
Dennis McTIgue (Committee Chair)
Purnima Kumar (Committee Member)
Ashok Kumar (Committee Member)
Kumar Subramanian (Committee Member)
Pages
64 p.
Subject Headings
Dental Care
Keywords
Ankylosis
;
Avulsion
;
Cytokines
;
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Ashley, A. (2012).
Immune Mediators in Gingival Crevicular Fluid as Predictors of Healing Outcomes in Re-Implanted Permanent Incisors – A Pilot Investigation
[Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1338376024
APA Style (7th edition)
Ashley, Amanda.
Immune Mediators in Gingival Crevicular Fluid as Predictors of Healing Outcomes in Re-Implanted Permanent Incisors – A Pilot Investigation.
2012. Ohio State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1338376024.
MLA Style (8th edition)
Ashley, Amanda. "Immune Mediators in Gingival Crevicular Fluid as Predictors of Healing Outcomes in Re-Implanted Permanent Incisors – A Pilot Investigation." Master's thesis, Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1338376024
Chicago Manual of Style (17th edition)
Abstract Footer
Document number:
osu1338376024
Download Count:
1,665
Copyright Info
© 2012, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.