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Cultivation of Nannochloropsis salina and Synechocystis sp. PCC6803 in Anaerobic Digestion Effluent for Nutrient Removal and Lipid Production

Cai, Ting
http://rave.ohiolink.edu/etdc/view?acc_num=osu1343748848

Abstract Details

2012, Master of Science, Ohio State University, Food, Agricultural and Biological Engineering.
Energy demands and climate change are among the major issues that challenge the development and sustainability of our society. Biofuels are one of the most promising substitute energy resources for fossil fuels because they are renewable and help reduce greenhouse gas emissions. Microalgal biomass is believed to be one of the most promising feedstocks for biofuel production. The water and nutrients required for the growth of microalgae could be supplied from wastewater. Anaerobic digestion (AD) is used worldwide to treat organic waste and produce biogas as a renewable energy. The digester liquor (AD effluent) is rich in nitrogen and phosphorus and is usually land applied as fertilizer. When there is not enough land within an economic transportation range, AD effluent needs specific treatment before being discharged. The abundant nutrients in AD effluent make it a good nutrient supplement for algae cultivation. Most of previous research about using AD effluent to grow algae has focused on freshwater algal species in digested wastewater from livestock production systems. However, there are no reports on the study of seawater algae Nannochloropsis salina and Synechocystis sp. PCC6803 in digested municipal wastewater. In this study, the growth rate, nutrient removal efficiency, and biomass and lipid productivity of N. salina and Synechocystis sp. PCC6803 were evaluated in both batch and semi-continuous cultivation systems using digested municipal wastewater as nutrient supplement. Batch results showed that N. salina grew well at AD effluent loadings of 3, 6, 12, and 18%. In contrast, the growth of Synechocystis sp. PCC6803 was gradually inhibited as the effluent loading increased from 3% to 24%, with a highest growth rate of 0.717 d-1 obtained at 3% loading. For both N. salina and Synechocystis sp. PCC6803, the nutrient removal efficiency decreased as the effluent loading increased, while N. salina was more efficient than Synechocystis sp. PCC6803 for nutrient removal. Although the highest biomass yield of Synechocystis sp. PCC6803 was 1.6 times that of N. salina, its lower lipid content resulted in lower lipid production. The optimal growth rate of N. salina and Synechocystis sp. PCC6803 was obtained at 6% and 3% effluent loading, respectively. The semi-continuous studies were carried out for 18 days using these optimal loading rates at different harvesting frequencies (1-, 2-, 3-day intervals) and harvesting ratios (25% and 50%). Compared to the batch study, both biomass and lipid productivity of N. salina were improved in the semi-continuous study at the same effluent loading. The highest lipid productivity of N. salina was 38.7 mg L-1 d-1 with 35.3 mg L-1 d-1 nitrogen removal and 3.8 mg L-1 d-1 phosphorus removal, when harvested every two days at a 50% harvesting ratio. The biomass productivity of Synechocystis sp. PCC6803 was 40% higher than that of N. salina, but its maximum lipid productivity (29.1 mg L-1 d-1), obtained at a 50% harvesting ratio and daily harvesting, was lower than that of N. salina.
Yebo Li (Advisor)
Jiyoung Lee (Committee Member)
Jay Martin (Committee Member)
115 p.
Agricultural Engineering
Nannochloropsis salina; Synechocystis sp. PCC6803; anaerobic digestion effluent; nutrient removal; lipid; microalgae; cyanobacteria

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Citations

  • Cai, T. (2012). Cultivation of Nannochloropsis salina and Synechocystis sp. PCC6803 in Anaerobic Digestion Effluent for Nutrient Removal and Lipid Production [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1343748848

    APA Style (7th edition)

  • Cai, Ting. Cultivation of Nannochloropsis salina and Synechocystis sp. PCC6803 in Anaerobic Digestion Effluent for Nutrient Removal and Lipid Production. 2012. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1343748848.

    MLA Style (8th edition)

  • Cai, Ting. "Cultivation of Nannochloropsis salina and Synechocystis sp. PCC6803 in Anaerobic Digestion Effluent for Nutrient Removal and Lipid Production." Master's thesis, Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1343748848

    Chicago Manual of Style (17th edition)

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This open access ETD is published by The Ohio State University and OhioLINK.